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Crotonylated and secreted proteins in Kcr effectors in Fusarium oxysporum

ABSTRACT: Crotonylated and secreted proteins in Kcr effectors in Fusarium oxysporum

INSTRUMENT(S):

ORGANISM(S): Fusarium Oxysporum F. Sp. Lini

TISSUE(S): Whole Body

SUBMITTER: Xianliang Ma

LAB HEAD: Ning Zhang

PROVIDER: PXD054345 | Pride | 2026-04-27

REPOSITORIES: pride

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			Action	DRS
	QE_HFX_20210303_50cm_60min_Kcro_1.raw	Raw
	QE_HFX_20210303_50cm_60min_Kcro_2.raw	Raw
	QE_HFX_20210303_50cm_60min_Kcro_3.raw	Raw
	txt.zip	Other

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Publications

Lysine crotonylation enhances fungal effector activity and promotes invasion of host plants.

Zhang Ning N Li Jingying J Wang Lei L Xu Jing J Du Juan J Liang Wenxing W

Plant communications 20251227 4

Phytopathogenic fungi secrete a large arsenal of effectors to facilitate host infection; however, the mechanisms regulating the activity of these virulence proteins remain elusive. Here, we show that at least 86 putative effectors in the fungal pathogen Fusariumoxysporum f. sp. lycopersici are crotonylated within their enzymatic domains, as revealed by proteomic analysis. Crotonylation of a chitinase and two plant cell wall-degrading enzymes by the fungus-specific crotonyltransferase FolRtt109 e ...[more]

PMID: 41456089

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Global Proteomic Analysis of Lysine crotonylation in The Plant Pathogen Botrytis cinerea

Project description:Lysine crotonylation (Kcr), a recently discovered post-translational modification, plays a key role in the regulation of diverse cellular processes. Botrytis cinerea is a destructive necrotrophic fungal pathogen distributed worldwide with broad ranging hosts. However, the functions of Kcr are unknown in B. cinerea or any other plant fungal pathogens. Here, we comprehensively evaluated the crotonylation proteome of B. cinerea and identified 3967 Kcr sites in 1041 proteins, of which contained in 9 types of modification motifs. Our results show that although the crotonylation were largely conserved, different organisms contained distinct crotonylated proteins with unique functions. Bioinformatic analysis demonstrated that the majority of crotonylated proteins were distributed in cytoplasm (35%), mitochondria (26%) and nucleus (22%). The identified proteins were found to be involved in various metabolic and cellular processes, such as cytoplasmic translation and structural constituent of ribosome. Particularly, 26 crotonylated proteins participated in the pathogenicity of B. cinerea, suggesting a significant role for Kcr in this process. Protein interaction network analysis demonstrated that a mass of protein interactions are regulated by crotonylation. These data represent the ﬁrst report of the crotonylome of B. cinerea and provide a good foundation for further explorations of the role of Kcr in plant fungal pathogens.

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Genome-wide transcriptional Responses to Fusarium oxysporum and Tomato mosaic virus in Tomato

Project description:The aim of this study was to compare the tomato global transcriptional profiles in response to host attack by ToMV and Fol in order to identify genomic differences and similarities in incompatible interactions between a foliar and a vascular pathogen. In order to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and Tomato Mosaic Virus (ToMV) a transcriptional analysis was performed. Tomato genes differentially expressed upon inoculation with Fol and ToMV were identified at 2 days post-inoculation, using an un-inoculated sample as reference.

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Tomato xylem sap proteome composition following endophyte- and R-gene mediated resistance towards Fusarium oxysporum f.sp. lycopersici inoculation.

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First comprehensive proteome analysis of lysine crotonylation in Streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts

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Global involvement of lysine crotonylation in protein modification and transcription regulation in rice

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