Proteomics

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Proteome profiling of trophoblasts reveals calcium involvement in syncytialisation and hormone production

ABSTRACT: Endocrine hormones of the placenta are essential for the maintenance of pregnancy, and disturbances in trophoblast integrity are associated with pregnancy complications. Here, we combine human trophoblast cell cultures, hormone assays, antibody-based detection methods and high-resolution mass spectrometry analyses to assess changes in cellular processes during syncytialisation. We monitored the changes in protein expression profiles during trophoblast syncytialization in an unbiased manner and show that the expression of numerous proteins is strongly altered. Among them are enzymes of the glucocorticoid and sex hormones synthesis pathways such as CYP19A1, CYP11A1, FDX1, HSD11B2 and HSD17B1, whose expression is strongly induced by syncytialization. The production of β-hCG, progesterone and estradiol increases several-fold during syncytialization, while the secretion and synthesis of ß-hCG and the expression of several syncytiotrophoblast markers show a clear calcium dependence

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Trophoblast

SUBMITTER: Claudia Fecher-Trost  

LAB HEAD: Claudia Fecher-Trost

PROVIDER: PXD054348 | Pride | 2025-05-07

REPOSITORIES: Pride

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WT_Ubi_Ca_Norm_DMSO_HBV2_ALG106_1.raw Raw
WT_Ubi_Ca_Norm_DMSO_HBV2_ALG106_1r.raw Raw
WT_Ubi_Ca_Norm_DMSO_HBV2_ALG106_2.raw Raw
WT_Ubi_Ca_Norm_DMSO_HBV2_ALG106_2r.raw Raw
WT_Ubi_Ca_Norm_DMSO_HBV2_ALG106_3.raw Raw
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Publications

The proteomic landscape of trophoblasts unravels calcium-dependent syncytialization processes and beta-chorionic gonadotropin (ß-hCG) production.

Gehl Anna-Lena AL   Klawitter Daniel D   Wissenbach Ulrich U   Cole Marnie M   Wesely Christine C   Löhr Heidi H   Weissgerber Petra P   Sota Adela A   Meyer Markus R MR   Fecher-Trost Claudia C  

Reproductive biology and endocrinology : RB&E 20250304 1

<h4>Background</h4>The syncytiotrophoblast (STB) layer of the placenta is formed by cell fusion of cytotrophoblasts, acts as a feto-maternal barrier, is required for the production of pregnancy hormones such as chorionic gonadotropin, estradiol and progesterone and is also responsible for feto-maternal mineral exchange such as calcium. Adequate mineral supply and placental hormone production are essential for the maintenance of pregnancy, and disturbances in trophoblast integrity are associated  ...[more]

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