Elucidation of the substrate specificity of the ABC transporter Tba uncovers an unsuspected role in the biosynthesis of glycopeptide antibiotics

ABSTRACT: In this project, the proximal proteins of the balhimycin transporter Tba in A. balhimycina were investigated in order to elucidate potential interaction partners. Tba is an ABC transporter, which is co-encoded with biosynthetic enzymes in the biosynthetic gene cluster of the glycopeptide antibiotic (GPA) balhimycin. Little is known about the interactions and regulatory roles of GPA exporters. Therefore, a proximity biotinylation approach was used to label proximal proteins prior to identification by mass spectrometry. Tba was expressed as a fusion protein with the promiscuous biotin ligase TurboID.

INSTRUMENT(S):

ORGANISM(S): Bacteria Amycolatopsis Balhimycina

SUBMITTER: Mirita Franz-Wachtel  

LAB HEAD: Samuel Wagner

PROVIDER: PXD054387 | Pride | 2025-05-07

REPOSITORIES: pride