Dataset Information

Elucidation of the substrate specificity of the ABC transporter Tba uncovers an unsuspected role in the biosynthesis of glycopeptide antibiotics

ABSTRACT: In this project, the proximal proteins of the balhimycin transporter Tba in A. balhimycina were investigated in order to elucidate potential interaction partners. Tba is an ABC transporter, which is co-encoded with biosynthetic enzymes in the biosynthetic gene cluster of the glycopeptide antibiotic (GPA) balhimycin. Little is known about the interactions and regulatory roles of GPA exporters. Therefore, a proximity biotinylation approach was used to label proximal proteins prior to identification by mass spectrometry. Tba was expressed as a fusion protein with the promiscuous biotin ligase TurboID.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacteria Amycolatopsis Balhimycina

SUBMITTER: Mirita Franz-Wachtel

LAB HEAD: Samuel Wagner

PROVIDER: PXD054387 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20240313_LC07_CO_1200EvSt_R01.raw	Raw
	20240313_LC07_CO_1200EvSt_R02.raw	Raw
	20240313_LC07_CO_1200EvSt_R03.raw	Raw
	20240313_LC07_CO_1200EvSt_R04.raw	Raw
	20240313_LC07_CO_1200EvSt_R06.raw	Raw

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Publications

Unveiling the substrate specificity of the ABC transporter Tba and its role in glycopeptide biosynthesis.

Gericke Nicola N Beqaj Dardan D Kronenberger Thales T Kulik Andreas A Gavriilidou Athina A Franz-Wachtel Mirita M Schoppmeier Ulrich U Harbig Theresa T Rapp Johanna J Grin Iwan I Ziemert Nadine N Link Hannes H Nieselt Kay K Macek Boris B Wohlleben Wolfgang W Stegmann Evi E Wagner Samuel S

iScience 20250303 4

Glycopeptide antibiotics (GPA) such as vancomycin are essential last-resort antibiotics produced by actinomycetes. Their biosynthesis is encoded within biosynthetic gene clusters, also harboring genes for regulation, and transport. Diverse types of GPAs have been characterized that differ in peptide backbone composition and modification patterns. However, little is known about the ATP-binding cassette (ABC) transporters facilitating GPA export. Employing a multifaceted approach, we investigated ...[more]

PMID: 40171492

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