Data-Independent Acquisition Mass Spectrometry as a Tool for Metaproteomics: Interlaboratory Comparison Using a Model Microbiome

ABSTRACT: Metaproteomics used bottom-up mass spectrometry to detect and quantify the proteins present in microbiome samples. Historically, the methodology of choice in these analyses has been Data-Dependent Acquisition mass spectrometry (DDA-MS). In this manuscript we see sought to evaluate the reproducibility and accuracy of data-independent acquisition mass spectrometry (DIA-MS) compared to DDA-MS on samples of known composition.

INSTRUMENT(S):

ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough Chlamydomonas Reinhardtii Unclassified Pseudomonas Fluorescens Group Escherichia Coli Nitrososphaera Viennensis Salmonella Phage P22 Chromobacterium Violaceum Bacteria Staphylococcus Aureus Stenotrophomonas Maltophilia Thermus Thermophilus Anthracocystis Tumefaciens Salmonella Typhimurium (strain Lt2 / Sgsc1412 / Atcc 700720) Archaea Escherichia Phage M13 Enterobacteria Phage Es18 Paracoccus Denitrificans Nitrosomonas Ureae Rhizobium Leguminosarum Cupriavidus Metallidurans Pseudomonas Denitrificans (nom. Rej.) Paraburkholderia Xenovorans Unclassified Bacillus Subtilis Group Nitrosospira Multiformis Enterobacteria Phage F2 Pseudomonas Cf. Pseudoalcaligenes Alteromonas Macleodii Roseobacter

DISEASE(S): Disease Free

SUBMITTER: Andrew Rajczewski  

LAB HEAD: Timothy J Griffin

PROVIDER: PXD054415 | Pride | 2024-12-19

REPOSITORIES: Pride