Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of targets of DBIC

ABSTRACT: Pyroptosis is a type of lytic cell death executed by members of the gasdermin family. It has been demonstrated that induction of pyroptosis can effectively inhibit tumor growth, which implicates a new strategy for clinical tumor therapy, especially for those tumors (such as melanoma) that are insensitive or resistant to the induction of apoptosis.By means of a chemical compound DBIC, designed and identified by our group recently, to induce GSDMC-mediated pyroptosis in melanoma cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Kejun Xu

LAB HEAD: Liu-zheng Wu

PROVIDER: PXD054435 | Pride | 2025-11-21

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	1_WLZ-LH118-G.xlsx	Xlsx
	WLZ-LH118-G_Slot2-1_1_26713.d.zip	Other
	checksum.txt	Txt

Items per page:

1 - 3 of 3

Similar Datasets

Nur77 induces GSDMC-mediated pyroptosis

Project description:Pyroptosis is a type of lytic cell death executed by members of the gasdermin family. It has been demonstrated that induction of pyroptosis can effectively inhibit tumor growth, which implicates a new strategy for clinical tumor therapy, especially for those tumors (such as melanoma) that are insensitive or resistant to the induction of apoptosis.By means of a chemical compound DBIC, designed and identified by our group recently, to induce GSDMC-mediated pyroptosis in melanoma cells.

2025-07-07 | GSE273365 | GEO

PRMT5-interacting proteins detected in Hey cells LC–MS

Project description:Our previous results indicated that PRMT5 is involved in CDDP resistance and may be regulated by phosphorylation modification. To further explore the mechanism of PRMT5 involved in CDDP resistance, PRMT5-interacting proteins were screened for potential PRMT5 kinases by mass spectrometry.

2025-03-06 | PXD061397 | Pride

Identification of METTL3 arginine methylation sites in CDDP-resistant Hey cells LC-MS

Project description:Previous studies have shown that nuclear localization of METTL3 is associated with CDDP resistance. To identify key PTMS involved in the nuclear localization of METTL3 in CDDP-resistant ovarian cancer cells, we assessed the global methylation of arginine, as well as METTL3 phosphorylation, acetylation, O-GlcNAcylation, and crotonylation in CDDP-resistant and parental ovarian cancer cells. The results showed that arginine methylation of METTL3 was increased in CDDP-resistant cell lines. To further identify the arginine methylation sites that may be involved in the METTL3 resistance mechanism, we identified the methylation modification sites of METTL3 by mass spectrometry.

2025-03-06 | PXD061401 | Pride

Identification of PRMT5 phosphorylation sites in Hey cells LC-MS

Project description:Previous studies have shown that PRMT5 undergoes various PTMs, including phosphorylation and arginine methylation. In our investigation of PRMT5's PTMs, we detected elevated phospho-Ser/Thr levels of PRMT5 in CDDP-resistant ovarian cancer cells Hey compared to their parental counterparts by co-immunoprecipitation of PRMT5. To further identify the phosphorylation sites that may be involved in the CDDP resistance mechanism, we identified the phosphorylation modification sites of PRMT5 by mass spectrometry.

2025-03-06 | PXD061400 | Pride

PRMT5-interacting proteins detected in Hey cells LC–MS

Project description:Through previous studies, we found that PRMT5 is regulated by phosphorylation modification and thus participates in tumor drug resistance. To further explore the mechanism of PRMT5 resistance, we screened potential PRMT5 kinases by mass spectrometry of proteins interacting with PRMT5

2025-02-22 | PXD058763 | Pride

hippocampus LC-MS experiment from Men1-CcKO mice and the controls

Project description:hippocampus dissection and conducted the LC-MS experiment in double-blind conditions

2024-07-03 | PXD042795 | Pride

Identification of ITM2B I115A binding proteins

Project description:ITM2B, a transmembrane protein, is frequently undergoing cleavage. We found site I115 is essential for ITM2B cleavage and ITM2BI115A exhibited resistant to cleavage. Here, we identified the interaction proteins with full-length ITM2B through analysis of mass spectrum in ITM2BI115A expressing 786-O cells.

2025-10-06 | PXD058330 | Pride

LC-MS of migrasomes and vesicles form human ccRCC 786-O cells

Project description:On one hand, we performed label-free quantitative proteomics to analyze migrasome proteins derived from 786-O cells vs ITM2B-knockdown 786-O cells.On the other hands,we isolated ITM2B truncation-containing vesicles and performed mass proteomic analysis to identify proteins that were carried by ITM2B truncation-containing vesicles. Through joint analysis of decreased proteins in ITM2B-knockdown migrasomes and proteins in vesicles, we discovered overlapping proteins, suggesting these proteins might be selectively sorted into migrasomes via ITM2B truncation-containing vesicles.

2025-10-06 | PXD054746 | Pride

Bacteriocin producing Enterococcus faecium

Project description:Proteomic Characterization of Bacteriocin-Producing Enterococcus faecium Strains from foodstuffs

2023-03-11 | PXD037241 | Pride

5-HT Reuptake Blockade Disrupts Genomic Stability-Based proteostasis to Induce Pyroptosis via Remodeling Histone Serotonylation [RNA-seq]

Project description:The dual challenges of limited therapeutic options caused by de novo or acquired resistance and psychological distress in melanoma patients necessitate innovative treatment strategies. In this study, we identify Paroxetine Hydrochloride (PH), an FDA-approved antidepressant, as a novel therapeutic agent for BRAFV600E-mutated melanoma, including BRAFi/MEKi-resistant cases. Furthermore, our findings unraveled that PH acts as an unrecognized pyroptosis inducer. By triggering pyroptosis, PH remodels the tumor microenvironment to synergize with anti-PD-1 therapy while maintaining a favorable safety profile. Mechanistically, PH impedes serotonin reuptake, leading to epigenetic reprogramming through reduced histone serotonylation at the promoters of DNA repair genes. The impairment of DNA damage repair pathways in turn triggers genomic instability, proteostasis imbalance, and endoplasmic reticulum stress, ultimately inducing pyroptosis. Our findings uncover the underlying mechanism by which 5-HT drives melanoma progression and highlight PH as a promising candidate with multiple clinical potentials for melanoma treatment.

2025-11-20 | GSE282621 | GEO