Dataset Information

LFQ proteomics miR-215 in Salmonella Typhimurium infected porcine intestinal cells

ABSTRACT: LFQ after transfection with miR-215 and/or infection with Salmonella Typhimurium

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Salmonellosis

SUBMITTER: Sara Zaldivar-Lopez

LAB HEAD: Juan Jose Garrido Pavon

PROVIDER: PXD054446 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	DBsearchps-copia.csv	Csv
	SaraZaldivar_sol437-69-21_215-2_14102021.raw	Raw
	SaraZaldivar_sol437-69-21_215-ST-2_14102021.raw	Raw
	SaraZaldivar_sol437-69-21_215-ST-3_14102021.raw	Raw
	SaraZaldivar_sol437-69-21_251-3_14102021.raw	Raw

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Publications

miR-215 Modulates Ubiquitination to Impair Inflammasome Activation and Autophagy During Salmonella Typhimurium Infection in Porcine Intestinal Cells.

Entrenas-García Carmen C Suárez-Cárdenas José M JM Fernández-Rodríguez Raúl R Bautista Rocío R Claros M Gonzalo MG Garrido Juan J JJ Zaldívar-López Sara S

Animals : an open access journal from MDPI 20250204 3

The host response to S. Typhimurium infection can be post-transcriptionally regulated by miRNAs. In this study, we investigated the role of miR-215 using both in vivo porcine infection models and in vitro intestinal epithelial cell lines. Several miRNAs were found to be dysregulated in the porcine ileum during infection with wild-type and SPI2-defective mutant strains of S. Typhimurium, with some changes being SPI2-dependent. Notably, miR-215 was significantly downregulated during ...[more]

PMID: 39943201

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Project description:Males and females exhibit differences in the incidence of many major diseases including auto-immune diseases, hepatocellular carcinoma, diabetes, and osteoporosis, which all have important inflammatory components in their etiology. Glucocorticoids are the primary physiological anti-inflammatory hormone in all mammals and synthetic derivatives of these hormones are widely prescribed as anti-inflammatory agents irrespective of gender. Surprisingly we report a marked sexually dimorphic regulation of gene expression by glucocorticoids in the rat liver. Eight distinct patterns of glucocorticoid regulated genes were identified and two revealed genes that respond to glucocorticoid treatment in both sexes in opposite directions (anti-correlated genes. We also identified gender-specific groups of genes regulated by glucocorticoids. Pathways analysis identified sex-specific glucocorticoid regulated gene expression in several canonical pathways that have been implicated in human disease in which disease susceptibility is sex-biased. For example, comparison of number of genes involved in inflammatory disorders between sexes, revealed 84 additional glucocorticoid responsive genes in the male rat. Our data suggests that glucocorticoids thorough sexually-dimorphic regulation of gene expression modulate gender specific homeostatic functions in male and female liver. Adult males and females adrenalectomized Sprague-Dawley rats (8-10 weeks of age) (females Distrous 1) were treated with vehicle (Phosphate Buffered Saline – 400 µl) or Dexamethasone (1mg/Kg i.p.) purchased from Steraloids (Newport, RI). Liver was harvested 6 hours after treatment and kept in RNA later solution at 4oC over night. Samples were frozen and stored at -80oC. Three replicate microarrays were completed for each group (male vehicle, male dexamethasone, female vehicle and female dexamethasone) for a total of 12 microarrays. Each microarray was hybridized using a pooled RNA sample, and each pool was created from equal amounts of total RNA from three independent RNA samples, representing individual animals (n=9 animals per group). This approach provides appropriate biological replication within the constraints of a limiting number of microarrays (C. Kendziorski, R. A. Irizarry, K. S. Chen, J. D. Haag, M. N. Gould, Proc Natl Acad Sci U S A 102, 4252 Mar 22, 2005).

Dataset Information

LFQ proteomics miR-215 in Salmonella Typhimurium infected porcine intestinal cells

Dataset's files

Publications

miR-215 Modulates Ubiquitination to Impair Inflammasome Activation and Autophagy During <i>Salmonella</i> Typhimurium Infection in Porcine Intestinal Cells.

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