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Trioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks

ABSTRACT: Here, we present a novel trioxane-based MS-cleavable homotrifunctional cross-linker TSTO, which can target three proximal lysine residues simultaneously. Owing to its unique structure and MS-cleavability, TSTO enables fast and unambiguous identification of cross-linked peptides using LC-MSn analysis. Importantly, we have demonstrated that the TSTO-based XL-MS platform is effective for mapping PPIs of protein complexes and cellular networks.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Clinton Yu

LAB HEAD: Lan Huang

PROVIDER: PXD054551 | Pride | 2025-05-30

REPOSITORIES: Pride

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			Action	DRS
	Compiled_InVivo.xlsx	Xlsx
	Compiled_Rpn1.xlsx	Xlsx
	OrbitrapLUMOS_20181016_01_UCI_01.raw	Raw
	OrbitrapLUMOS_20181016_02_UCI_02.raw	Raw
	OrbitrapLUMOS_20181017_05_UCI_03.raw	Raw

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Trioxane-based MS-cleavable Cross-linking Mass Spectrometry for Profiling Multimeric Interactions of Cellular Networks

Project description:Here, we present a novel trioxane-based MS-cleavable homotrifunctional cross-linker TSTO, which can target three proximal lysine residues simultaneously. Owing to its unique structure and MS-cleavability, TSTO enables fast and unambiguous identification of cross-linked peptides using LC-MSn analysis. Importantly, we have demonstrated that the TSTO-based XL-MS platform is effective for mapping PPIs of protein complexes and cellular networks.

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