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Proteomic analysis of human gingival fibroblast secretomes from healthy and periodontitis patients

ABSTRACT: The objective of this preliminary study was to compare the secretomes of human gingival fibroblasts from healthy and periodontitis patients in the context of wound healing.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Periodontitis

SUBMITTER: Siddharth Shanbhag

LAB HEAD: Siddharth Shanbhag

PROVIDER: PXD054664 | Pride | 2025-05-07

REPOSITORIES: Pride

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	D1.msfView	Msf
	D1.raw	Raw
	D10_ny.raw	Raw
	D11.raw	Raw
	D13.raw	Raw

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Publications

Secretomes of Gingival Fibroblasts From Periodontally Diseased Tissues: A Proteomic Analysis.

Smedås Anne Kari AK Paris Lovise Gangeskar LG Al-Sharabi Niyaz N Kristoffersen Einar K EK Sanz Mariano M Mustafa Kamal K Bunæs Dagmar Fosså DF Shanbhag Siddharth S

Clinical and experimental dental research 20250201 1

<h4>Objective</h4>Cell secretomes represent a promising strategy for periodontal and bone regeneration. The objective of this study was to characterize the secretome of human gingival fibroblasts (GF) from periodontally diseased tissues (GF-perio) using proteomics.<h4>Materials and methods</h4>Conditioned media of GF-perio from periodontitis patients (n = 6, 48-h serum-free culture) were subjected to liquid chromatography with tandem mass spectrometry. Global profiles, differentially expressed p ...[more]

PMID: 39988729

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Transcriptome of healthy gingival tissue in aggressive periodontitis

Project description:This study evaluated the transcriptome of healthy gingival tissue in patients with a history of generalized aggressive periodontitis (GAgP) and chronic periodontitis (CP) and in subjects with no history of periodontitis (H), using microarray analysis.

2017-03-29 | GSE79705 | GEO

Transcriptome analysis of chronic periodontitis patients' gingival tissue

Project description:We examined the molecular and cellular mechanism for chronic periodontitis in patients' gingival tissue by whole transcriptome sequencing.

2016-08-18 | GSE80715 | GEO

Lipidomic analysis reveals altered lipid profiles of clinically healthy and periodontitis-affected gingival biopsies

Project description:<p><strong>AIM:</strong> The role of lipids in periodontitis has not been well studied. Thus, this study aimed to explore periodontitis-associated lipid profile changes and identify differentially expressed lipid metabolites in gingival tissues.</p><p><strong>MATERIALS AND METHODS:</strong> Gingival tissues from 38 patients with periodontitis (periodontitis group) and 38 periodontally healthy individuals (control group) were collected. A UHPLC-QTOF-MS-based non-targeted metabolomics platform was used to identify and compare the lipid profiles of the two groups. The distribution and expression of related proteins were subsequently analyzed via immunohistochemistry to further validate the identified lipids.</p><p><strong>RESULTS:</strong> Lipid profiles significantly differed between the two groups, and 20 differentially expressed lipid species were identified. Lysophosphatidylcholines (lysoPCs), diacylglycerols (DGs) and phosphatidylethanolamines (PEs) were significantly upregulated, while triacylglycerols (TGs) were downregulated in the periodontitis group. Moreover, the staining intensity of ABHD5/CGI-58, secretory phospholipase A2 (sPLA2), and sPLA2-IIA was significantly stronger in the gingival tissues of patients with periodontitis than in those of healthy controls.</p><p><strong>CONCLUSIONS:</strong> LysoPCs, DGs, and PEs were significantly upregulated, whereas TGs were downregulated in gingival tissues of patients with periodontitis. Correspondingly, the immunohistochemical staining of ABHD5/CGI-58, sPLA2 and sPLA2-IIA in gingival tissues was consistent with the downstream production of lipid classes (lysoPCs, TGs and DGs).</p>

2022-08-19 | MTBLS4684 | MetaboLights

Altered gene expressions of leukocyte transendothelial migration and cell communication pathways in periodontitis-affected gingival tissues

Project description:Gene expressions relate to the pathogenesis of periodontitis and have a crucial role in local tissue destruction and susceptibility to the disease. The aims of the present study were to explore comprehensive gene expressions/transcriptomes in periodontitis-affected gingival tissues, and to identify specific biological processes. The purpose of the present study was 1) to compare comprehensive gene expression/transcriptomes of periodontitis-affected gingival tissues with those of healthy tissues by using microarray and data mining technologies, and 2) to analyze significantly differentially expressed genes which belong to pathological pathways in periodontitis by qRT-PCR. Two distinct gingival samples including healthy and periodontal-affected gingiva were taken from 3 patients with severe chronic periodontitis. Total RNAs from 6 gingival tissue samples were used for microarray and data-mining analyses. Comparisons, gene ontology, and pathway frequency analyses were performed and identified significant biological pathways in periodontitis. Quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR) analyse using 14 chronic periodontitis patients including 3 patients listed above and 14 healthy individuals showed 9 differentially expressed genes in leukocyte migration and cell communication pathways.

2010-08-13 | E-GEOD-23586 | biostudies-arrayexpress

Transcription profiling of gingival tissue from patients with periodontitis: Bacterial Correlates of Gingival Gene Expression

Project description:We investigated the association between subgingival bacterial profiles and gene expression patterns in gingival tissues of patients with periodontitis. A total of 120 patients undergoing periodontal surgery contributed with a minimum of two interproximal gingival papillae (range 2-4) from a maxillary posterior. Prior to tissue harvesting, subgingival plaque samples were collected from the mesial and distal aspects of each papilla. Gingival tissue RNA was extracted, reverse-transcribed, labeled, and hybridized with whole-genome microarrays (310 in total)

2009-12-21 | E-GEOD-16134 | biostudies-arrayexpress

Transcriptomes in Healthy and Diseased Gingival Tissues

2008-12-22 | GSE10334 | GEO

Fibroblast VEGF-receptor 1 expression as molecular target in periodontitis

Project description:To identify gene expression profiles in those periodontitis-associated fibroblasts (PAFs) versus normal gingival fibroblasts to determine their molecular repertoire, and exploit it for therapeutic intervention. We collected RNA from 4 paired PAF and non PAF from patients with periodontitis

2015-03-11 | E-GEOD-66771 | biostudies-arrayexpress

MicroRNA profiling in gingival crevicular fluid of periodontitis

Project description:We profiled miRNAs in gingival crevicular fluid (GCF) by a PCR-based method that yielded quantitative measures of more than 600 miRNAs. We found that miRNA profiles in GCF of periodontitis patients are distinct from those of healthy controls.

2017-08-11 | GSE89081 | GEO

Gingival transcriptomics phases of periodontitis lesions in nonhuman primate model

Project description:We used a nonhuman primate (NHP) model of ligature-induced periodontitis to identify gingival transcriptome changes associated with aging during the phases of periodontitis lesions (initiation, progression, and resolution). Four age groups of nonhuman primate were studied: Young (<3 years of age); Adolescent (3 to 7 years), Adult (12 to 15 years), and Aged (17-23 years)

2021-07-22 | GSE180588 | GEO

Transcriptomes in Healthy and Diseased Gingival Tissues

Project description:We examined gene expression signatures in healthy and diseased gingival tissues in 90 patients. Analysis of the gingival tissue transcriptome in states of periodontal health and disease may reveal novel insights of the pathobiology of periodontitis. Keywords: gingival tissue disease state analysis Ninety non-smokers, 63 with chronic and 27 with aggressive periodontitis, each contributing with >=2 “diseased” interproximal papillae (with bleeding on probing, probing pocket depth >=4mm, and clinical attachment loss >=3mm) and a “healthy” papilla, if available (no BoP, PPD<=4mm and CAL<=2mm). RNA was extracted, amplified, reverse transcribed, labeled, and hybridized with AffymetrixU133Plus2.0 arrays. Transcriptome analysis was conducted on a total of 247 samples (from 183 diseased and 64 healthy sites).

2008-12-22 | E-GEOD-10334 | biostudies-arrayexpress

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