Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional properties of Candida albicans extracellular vesicles released in the presence of the antifungal drugs amphotericin B, fluconazole and caspofungin

ABSTRACT: Identification of proteins contained in extracellular vesicles of Candida albicans 10231 released in the presence of the antifungal drugs amphotericin B (AMB), fluconazole (FLU) and caspofungin (CASP). We acknowledge the Proteomics and Mass Spectrometry Core Facility of the Malopolska Centre of Biotechnology for protein identification. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464). Data set is related to publication DOI 10.1099/mic.0.001565.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Justyna Karkowska-Kuleta

LAB HEAD: Justyna Karkowska-Kuleta

PROVIDER: PXD054683 | Pride | 2025-06-06

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	EV_AMB.mgf	Mgf
	EV_AMB.mzid	Mzid
	EV_AMB.raw	Raw
	EV_CASP.mgf	Mgf
	EV_CASP.mzid	Mzid

Items per page:

1 - 5 of 12

Similar Datasets

Extracellular vesicles of probiotic microorganism Streptococcus salivarius

Project description:Identification of proteins from extracellular vesicles isolated from Streptococcus salivarius. This work was financially supported by the National Science Centre, Poland (grant number 2021/43/D/NZ6/01464).

2023-05-24 | PXD039138 | Pride

Characteristic of extracellular vesicles of probiotic yeasts Saccharomyces boulardii

Project description:Identifictaion of proteins from extracellular vesicles of probiotic yeasts Saccharomyces boulardii. This work was financially supported by the National Science Centre, Poland (grant number 2021/43/D/NZ6/01464).

2023-05-24 | PXD039137 | Pride

Extracellular vesicles of Lactiplantibacillus plantarum PCM 2675

Project description:Identification of proteins contained in extracellular vesicles of Lactiplantibacillus plantarum PCM 2675. Dataset is related to publication http://dx.doi.org/10.20517/evcna.2024.49. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464).

2024-11-07 | PXD048874 | Pride

Extracellular vesicles of Lacticaseibacillus rhamnosus PCM 489

Project description:Identification of proteins contained in extracellular vesicles of Lacticaseibacillus rhamnosus PCM 489. Dataset is related to publication http://dx.doi.org/10.20517/evcna.2024.49. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464).

2024-11-07 | PXD048876 | Pride

3D model of human epidermis exposed to Staphylococci reveals keratinocyte responses unique to atopy-associated S. aureus challenge.

Project description:The interplay between microbiota and skin cells is a key factor underlying the pathophysiology of this barrier organ. Keratinocytes, the main components of the epidermis, are crucial for skin barrier function, but their responses to different bacteria are not well understood. We present a high-throughput analysis of the transcriptome of human epidermal-like organoids exposed to two vastly different bacterial skin colonists: Staphylococcus epidermidis and Staphylococcus aureus, which are commonly associated with mostly positive and negative interactions with the host, respectively. We observed partly overlapping but predominantly divergent epidermal changes in response to both bacteria. Among the genes most affected by S. aureus, those related to lipid metabolism were predominant. This work was supported by grants from the Polish National Science Center (UMO-2014/12/W/NZ6/00454 and 2019/35/B/NZ6/03357) and by a grant for research projects for Young Scientists and PhD students from the Jagiellonian University (N19/MNS/000020). The open-access publication has been supported by the Faculty of Biochemistry, Biophysics and Biotechnology under the Strategic Programme Excellence Initiative at Jagiellonian University in Krakow, Poland.

2025-03-13 | GSE276844 | GEO

Transcription profiling of Candida albicans biofilm exposed to amphotericin B, fluconazole or two concentrations of caspofungin

Project description:30h of growth biofilms in microfermentors were exposed to caspofungin 0.5 µg/ml, caspofungin 5 µg/ml, fluconazole 80 µg/ml or amphotericin B 8 µg/ml. Control and antifungal-exposed biofilms were recovered at t= 0, 30, 60 and 120 min post exposure to antifungal.

2010-03-02 | E-MEXP-2436 | biostudies-arrayexpress

The proteomic response of Aspergillus fumigatus wildtype and rtaA knockout strain to Amphotericin B

Project description:Amphotericin B (AmB) and AmBisome are vital treatments for invasive fungal infections, particularly invasive aspergillosis caused by Aspergillus fumigatus spores. However, increasing resistance to AmB in clinical isolates of A. fumigatus is a growing concern. Despite this, the mechanisms of AmB resistance in A. fumigatus remain unclear. In this study, we conducted a proteomic analysis of A. fumigatus exposed to sublethal concentrations of AmB and AmBisome, identifying proteins that were strongly induced by these drugs. One of the most upregulated proteins was RtaA, which encodes a member of the RTA-like protein family, that comprises conserved fungal membrane proteins with putative functions as transporters or translocases. We found that deleting rtaA led to increased polyene sensitivity and overexpression to modest resistance and is mainly located in plasma and partially vacuolar membranes. Interestingly, rtaA expression was only induced by exposure to AmB and nystatin, but not by other antifungals such as itraconazole and caspofungin. Deletion of rtaA did not significantly change the ergosterol content of A. fumigatus, but decreased fluorescence intensity of the sterol-binding stain filipin. This suggests that RtaA is involved in sterol and lipid trafficking, possibly by transporting the target ergosterol from or to lipid droplets. These findings reveal a novel polyene resistance mechanism that warrants further investigation to determine its exact mechanism and importance in clinical resistant isolates of A. fumigatus.

2025-05-05 | PXD056876 | Pride

Whole genome sequencing of fluconazole tolerant adaptors derived from exposure to echinocandins in Candida albicans

Project description:Candida albicans lab strain SC5314 was spread on YPD plates supplemented with echinocandins (caspofungin, anidulafungin, micafungin, rezafungin). The adaptors were tested for tolrance to fluconazole. Randomly 43 tolerant adaptors, as well as the parent SC5314, were sequenced.

2023-07-11 | E-MTAB-12449 | biostudies-arrayexpress

Assessing the effect of Flubendazole on Jurkat T cells

Project description:Human Junket T cells grown to confluence in 100 mm plates in complete medium were incubated with 10 µM Flubendazole (FLU) or vehicle for 4 h. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, cat. 74004) with minor modification. Samples were submitted to the UNSW Ramaciotti Centre for sequencing and gene expression analysis was carried out.

2023-09-03 | GSE220995 | GEO

On the way to elucidate the effects of cannabidiol (CBD) on living organisms - assessment of changes in the global transcription profile of kidney in a mouse model

Project description:Cannabidiol (CBD) is a phytocannabinoid known for its anti-inflammatory, immunomodulatory, antioxidant, anxiolytic, antipsychotic, and anticonvulsant effects. The systemic administration of CBD also has the potential to impact kidney function, either beneficially or adversely. This study aims to examine the effects of CBD on behavior, immune system activity, and stress levels, and to determine its impact on transcriptomic alterations in selected organs of mice. (Study funded by the National Science Centre, Poland; project no. 2020/39/O/NZ9/00821)

2024-11-26 | GSE275998 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data