Blue Native PAGE co-fractionation of purified human endosomes

ABSTRACT: EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified by the modified Endo-IP protocol were subjected to Blue Native PolyAcrylamide Gel Electrophoresis (BN-PAGE) co-fractionation to identify possible protein complexes based on correlation profiling.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Miguel A. Gonzalez-Lozano  

LAB HEAD: J. Wade Harper

PROVIDER: PXD054684 | Pride | 2025-03-29

REPOSITORIES: Pride