Dataset Information

Quantitative differences in rumen epithelium proteins, as well as body composition and metabolizable energy intake in lambs fed a low or high energy diet.

ABSTRACT: The rumen is a major the site of digestion and absorption of energy predominantly in the form of short chain fatty acids as well as amino acids and simple carbohydrates. The research sought to determine if diets high or low in ME were associated with quantitative changes in the membrane bound proteins that transport and metabolise nutrients in the rumen epithelium (RE). The comprehensive assignment of proteins (>4000) to cellular location and quantification of differences in protein abundance will progress the annotation and understanding of protein functions in the ruminant genomes.

INSTRUMENT(S):

ORGANISM(S): Ovis Aries

TISSUE(S): Rumen Epithelium, Epithelial Cell

DISEASE(S): Disease Free

SUBMITTER: Karthik Shantharam kamath

LAB HEAD: Dr Jude Bond

PROVIDER: PXD054700 | Pride | 2026-02-09

REPOSITORIES: Pride

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Publications

Quantitative differences in rumen epithelium proteins and detection of lysine acetylation in lambs fed a low or high metabolizable energy diet.

Bond Judith J JJ Hudson Nicholas J NJ Kamath Karthik S KS Wheeler David D Woodgate Serey S Oddy Victor H VH

Journal of animal science 20250101

Thirty-six Merino wethers (10-mo-old) were fed ad libitum for 30 d 2 diets;1) low metabolizable energy diet (LME; 30% lucerne: 70% cereal chaff) and 2) high ME diet (HME; 40% rolled barley grain: 50% lucerne: 10% cereal chaff). Effects of diet on dry matter intake (DMI), ME intake (MEI), liveweight (LWT), average daily gain (ADG), carcass lean or fat gain, liver and empty rumen weight, and plasma metabolites were analyzed. A membrane-enriched protein fraction of rumen epithelium (RE) isolated en ...[more]

PMID: 39907549

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