Interactome of chromatin-associated RNA Polymerase II from U2OS cells

ABSTRACT: To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative mass spectrometry. U2OS cells expressing no HA-tagged protein were used for comparison.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Pranjali Bhandare  

LAB HEAD: Elmar Wolf

PROVIDER: PXD054701 | Pride | 2024-12-20

REPOSITORIES: Pride