Dataset Information

Initial effects of BBN treatment on mouse bladder

ABSTRACT: Characterizing the initial stages of oncogenic transformation in bladder cancer. Littermate male mice were transferred in single-use plastic cages in order to be treated with the BBN carcinogen. The treatment dose used in this work was 0.05% BBN delivered in drinking water for up to 12 days. Bladder samples were collected at day 1, 4, 8 and 12 from treated mice along with the age-matched controls (non-treated). The drinking water was changed every fourth day. The weights of the mice and BBN water intake were measured every four days and mice were checked regularly for any signs of discomfort and pain.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bladder Organ, Bladder

SUBMITTER: Thomas Aga Legøy

LAB HEAD: Simona Chera

PROVIDER: PXD054745 | Pride | 2025-03-12

REPOSITORIES: Pride

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			Action	DRS
	572925_ea11984_ThomMouse.mzIdentML	Mzid
	572926_ea11983_ThomMouse.mzIdentML	Mzid
	572927_ea11982_ThomMouse.mzIdentML	Mzid
	572928_ea11981_ThomMouse.mzIdentML	Mzid
	572929_ea11980_ThomMouse.mzIdentML	Mzid

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Publications

Mapping the initial effects of carcinogen-induced oncogenic transformation in the mouse bladder.

Hossain Md Kaykobad MK Unger Lucas L Larsen Ulrik U Altankhuyag Altanchimeg A Legøy Thomas Aga TA Paulo Joao A JA Vethe Heidrun H Ghila Luiza L

Experimental cell research 20250221 2

Characterizing the initial stages of oncogenic transformation allows the identification of tumor-promoting processes before the inherent clonal selection of the aggressive clones. Here, we used global proteomics, genetic cell tracing, and immunofluorescence to dynamically map the very early stages of cancer initiation in a mouse model of bladder cancer. We observed a very rapid and incremental proteome dysregulation, with changes in the energy metabolism, proliferation and immune signatures domi ...[more]

PMID: 39988124

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