LC-MS of migrasomes and vesicles form human ccRCC 786-O cells

ABSTRACT: On one hand, we performed label-free quantitative proteomics to analyze migrasome proteins derived from 786-O cells vs ITM2B-knockdown 786-O cells.On the other hands,we isolated ITM2B truncation-containing vesicles and performed mass proteomic analysis to identify proteins that were carried by ITM2B truncation-containing vesicles. Through joint analysis of decreased proteins in ITM2B-knockdown migrasomes and proteins in vesicles, we discovered overlapping proteins, suggesting these proteins might be selectively sorted into migrasomes via ITM2B truncation-containing vesicles.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Rcc 786-o Cell, Cell

DISEASE(S): Kidney Cancer

SUBMITTER: Qitao Chen  

LAB HEAD: Qi-tao Chen

PROVIDER: PXD054746 | Pride | 2025-10-06

REPOSITORIES: Pride