Immunoprecipitation of INSIG1/2 and mass spectrometry analysis

ABSTRACT: Huh7 cells with or without expression of Flag-INSIG1/2 were treated with or without high glucose for 2 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-INSIG1 were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Brilliant Blue. Selected peptide hits of proteins associated with Flag-INSIG1/2.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: yuran duan  

LAB HEAD: daqian Xu

PROVIDER: PXD054753 | Pride | 2025-12-26

REPOSITORIES: Pride