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Immunoprecipitation of INSIG1/2 and mass spectrometry analysis

ABSTRACT: Huh7 cells with or without expression of Flag-INSIG1/2 were treated with or without high glucose for 2 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-INSIG1 were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Brilliant Blue. Selected peptide hits of proteins associated with Flag-INSIG1/2.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: yuran duan

LAB HEAD: daqian Xu

PROVIDER: PXD054753 | Pride | 2025-12-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Q3_FW2022-002-290_INSIG1.msf	Msf
	Q3_FW2022-002-290_INSIG1.raw	Raw
	Q3_FW2022-002-290_INSIG2.msf	Msf
	Q3_FW2022-002-290_INSIG2.raw	Raw
	checksum.txt	Txt

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