Dataset Information

MS-based proteomic characterization of pacmanvirus lostcity virions

ABSTRACT: The microbial sampling of submarine hydrothermal vent remains challenging, with even fewer studies focused on viruses. Here we report the first isolation of a eukaryotic virus from the Lost City hydrothermal field, by co-culture with the laboratory host Acanthamoeba castellanii. This virus, named pacmanvirus lostcity, is closely related to previously isolated pacmanviruses (strains A23 and S19), clustering in a divergent clade within the long-established family Asfarviridae. Its icosahedral particles are 200 nm in diameter, with an electron-dense core surrounded by an inner membrane. Its genome of 395,708 bp (33% G+C) is predicted to encode 473 proteins. However, besides these standard properties, pacmanvirus lostcity was found associated with a new type of selfish genetic element (pltv), 7 kb in length, whose architecture and gene content are reminiscent of those of transpovirons, hitherto specific to the family Mimiviridae. We performed MS-based proteomic analyses of purified virions to characterize its protein content.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Asfarviridae Acanthamoeba Castellanii Str. Neff

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD054858 | Pride | 2025-05-07

REPOSITORIES: Pride

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Pacmanvirus isolated from the Lost City hydrothermal field extends the concept of transpoviron beyond the family Mimiviridae.

Santini Sébastien S Lartigue Audrey A Alempic Jean-Marie JM Couté Yohann Y Belmudes Lucid L Brazelton William J WJ Lang Susan Q SQ Claverie Jean-Michel JM Legendre Matthieu M Abergel Chantal C

The ISME journal 20250101 1

The microbial sampling of submarine hydrothermal vents remains challenging, with even fewer studies focused on viruses. Here we report what is to our knowledge the first isolation of a eukaryotic virus from the Lost City hydrothermal field, by co-culture with the laboratory host Acanthamoeba castellanii. This virus, named pacmanvirus lostcity, is closely related to previously isolated pacmanviruses (strains A23 and S19), clustering in a divergent clade within the long-established family Asfarvir ...[more]

PMID: 39789911

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			Action	DRS
	Clone1-R1.mgf	Mgf
	Clone1-R1.raw	Raw
	Clone1-R2.mgf	Mgf
	Clone1-R2.raw	Raw
	Clone2-R1.mgf	Mgf