Dataset Information

APEX2-MCM2 proximity labeling with high-resolution quantitative mass spectrometry in ESC

ABSTRACT: Through the APEX2 technique, we identified MCM2-interacting proteins associated with the replication initiation complex. After excluding proteins from the negative controls, we detected a substantial number of interacting proteins in the positive groups of both cell types. This research establishes a basis for further study on the component proteins of the conserved DNA replication initiation complex and the transient regulatory network involving its proximal proteins.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Jiaqi Zhou

LAB HEAD: Haiyun Gan

PROVIDER: PXD054939 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	20220614-YST-1-SYN-H.raw	Raw
	20220614-YST-10-SYN-H-.raw	Raw
	20220614-YST-11-CON-H.raw	Raw
	20220614-YST-12-CON-H-.raw	Raw
	20220614-YST-2-SYN-H-.raw	Raw

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Publications

Identification of MCM2-Interacting Proteins Associated with Replication Initiation Using APEX2-Based Proximity Labeling Technology.

Yao Sitong S Yue Zhen Z Ye Shaotang S Liang Xiaohuan X Li Yugu Y Gan Haiyun H Zhou Jiaqi J

International journal of molecular sciences 20250125 3

DNA replication is a crucial biological process that ensures the accurate transmission of genetic information, underpinning the growth, development, and reproduction of organisms. Abnormalities in DNA replication are a primary source of genomic instability and tumorigenesis. During DNA replication, the assembly of the pre-RC at the G1-G1/S transition is a crucial licensing step that ensures the successful initiation of replication. Although many pre-replication complex (pre-RC) proteins have bee ...[more]

PMID: 39940790

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