APEX2-MCM2 proximity labeling with high-resolution quantitative mass spectrometry in ESC

ABSTRACT: Through the APEX2 technique, we identified MCM2-interacting proteins associated with the replication initiation complex. After excluding proteins from the negative controls, we detected a substantial number of interacting proteins in the positive groups of both cell types. This research establishes a basis for further study on the component proteins of the conserved DNA replication initiation complex and the transient regulatory network involving its proximal proteins.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Jiaqi Zhou  

LAB HEAD: Haiyun Gan

PROVIDER: PXD054939 | Pride | 2025-05-07

REPOSITORIES: Pride