Lysosomal proteome characterization during TRPML1 activation by MLSA5 in human cancer cells

ABSTRACT: To capture the changes in the lysosomal proteome upon TRPML1 activation, achieved using a small molecule agonist MLSA5, human cancer cells, with or without gene-editing, stably expressing TMEM192-mRFP-3xHA (Lyso-IP), were treated with MLSA5 and Lyso-IP samples were prepared and processed for TMT-MS analysis. Each condition was performed in triplicate.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Chan Lee  

LAB HEAD: J. Wade Harper

PROVIDER: PXD054968 | Pride | 2025-04-22

REPOSITORIES: Pride