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Charting the dynamic trophoblast plasma membrane identifies LYN as a functional regulator of syncytialization (supplemental)

ABSTRACT: The differentiation of placental cytotrophoblasts (CTBs) into the syncytiotrophoblast (STB) layer results in significant remodeling of the plasma membrane proteome. Here, we use a peroxidase-catalyzed proximity labeling strategy to quantitatively map the dynamic plasma membrane proteomes of CTBs and STBs. Coupled with mass spectrometry-based proteomics, we identify hundreds of plasma membrane proteins and observe quantitative changes in protein abundance throughout differentiation, including the upregulation of the plasma membrane-localized non-receptor tyrosine kinase LYN. We show that both siRNA-mediated knockdown and small molecule inhibition of LYN kinase function impairs CTB fusion and abrogates the expression of syncytialization markers, presenting a function for LYN outside of its canonical role in immunological signaling. Our results demonstrate the use of the proximity labeling platform to discover functional regulators within the plasma membrane and provide new avenues to regulate trophoblast differentiation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Placenta, Mononuclear Cytotrophoblast Cell

SUBMITTER: Abigail Reeves

LAB HEAD: Dr. Mia L Huang

PROVIDER: PXD055086 | Pride | 2025-10-02

REPOSITORIES: Pride

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			Action	DRS
	MS221065_MH_AR_AER_B1_155.msf	Msf
	MS221065_MH_AR_AER_B1_155.mzML	Mzml
	MS221065_MH_AR_AER_B1_155.mzid.gz	Mzid
	MS221065_MH_AR_AER_B1_155.raw	Raw
	checksum.txt	Txt

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Charting the dynamic trophoblast plasma membrane identifies LYN as a functional regulator of syncytialization (supplemental)

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