Dataset Information

Proteomic profiling in Saccharomyces cerevisiae with abrogated binding of Nab3 to PIC2

ABSTRACT: Whole-proteome profiling enabled us to compare proteomic differences between a Saccharomyces cerevisiae parental strain (PIC2-GFP) and two derived mutants lacking Nab3 RNA-binding sites in PIC2. Previous experiments had established that disrupting Nab3 binding to PIC2 while maintaining Nrd1 binding to the same transcript led to severe growth defects, larger cell size, delayed cell cycle and resistance to oxidative stress. Following characterization of a PIC2-overexpressing mutant we established that these phenotypes were not related to Pic2 overabundance. Therefore, the purpose of this experiment was to determine (i) whether the proteomic signature of the mutant lacking Nab3 RNA-binding sites in PIC2 could account for the observed phenotypes and (ii) if the proteins that were differentially expressed in such mutant were targeted by Nab3 and Nrd1.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Sofia Esteban Serna

LAB HEAD: Sander Granneman

PROVIDER: PXD055087 | Pride | 2025-12-01

REPOSITORIES: Pride

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Publications

A transcription termination mechanism for maintaining homogeneous protein expression.

Esteban-Serna Sofia S Widén Tove T Gwynne Mags M Farquhar Iseabail I Duchen Michael R MR Swain Peter S PS Granneman Sander S

Nucleic acids research 20251101 21

Premature transcription termination is a critical mechanism for repressing harmful, unproductive transcription. In Saccharomyces cerevisiae, premature termination by the Nrd1-Nab3-Sen1 (NNS) complex also triggers decay of mRNAs induced to counteract stress. However, why mRNAs might be upregulated and simultaneously targeted for degradation remains unclear. Our work reveals a novel role for NNS as a gene expression noise suppression system. Single-cell analyses demonstrate that targeted disruptio ...[more]

PMID: 41261861

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Dataset Information

Proteomic profiling in Saccharomyces cerevisiae with abrogated binding of Nab3 to PIC2

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Publications

A transcription termination mechanism for maintaining homogeneous protein expression.

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