Dataset Information

Human sperm LC-MS/MS analysis after various selection techniques

ABSTRACT: To optimize semen samples from patients with high DNA fragmentation through various sperm selection methods, and to compare the differences in sperm protein expression among these methods.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Sperm

SUBMITTER: Shen Zhang

LAB HEAD: Shen Zhang

PROVIDER: PXD055160 | Pride | 2026-02-23

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 10

Publications

Female reproductive tract-on-a-chip for selecting sperm with ultra-low DNA fragmentation index.

Dai Jing J Shan Han H Gu Yifan Y Zheng Mingde M Lou Li L Xie Pingyuan P Zhang Shen S Sun Zheng Z Gong Fei F Lu Guangxiu G Lin Ge G Chen Zeyu Z

Microsystems & nanoengineering 20260214 1

High levels of sperm DNA fragmentation index (DFI) represent a critical factor in male infertility, with detrimental effects on embryonic development and offspring well-being. However, selecting sperm with low DFI remains a tremendous challenge. Here, we present an organ-level selection strategy capable of isolating spermatozoa with ultra-low DFI, achieving a remarkable reduction to 0.13% compared to 34.57% observed in raw semen. We design and fabricate a female reproductive tract (FRT)-on-a-chi ...[more]

PMID: 41690931

Similar Datasets

Project description:It is now well established that mature mammalian spermatozoa carry a population of mRNA molecules, at least some of which are transferred to the oocyte at fertilisation. However, the function of the sperm transcriptome remains largely unclear. To shed light on the evolutionary conservation of this feature of sperm biology, we analysed highly purified populations of mature sperm from the fruitfly, Drosophila melanogaster. As with mammalian sperm, we found a consistently enriched population of mRNA molecules that are not likely to be derived from contaminating somatic cells or immature sperm. Using tagged transcripts for three of the spermatozoal mRNAs, we demonstrate that they are transferred to the oocyte at fertilisation and can be detected at least until the onset of zygotic gene expression. We find a remarkable conservation in the functional annotations associated with fly and human spermatozoal mRNAs, in particular a highly significant enrichment for transcripts encoding Ribosomal Proteins. The identification of a conserved set of spermatozoal transcripts opens the possibility of using the power of Drosophila genetics to address the function of this enigmatic class of molecules. RNA extracted from three biological replicates of purified sperm (Sperm rep1, Sperm rep2 and Sperm rep3) was used as a template for oligo-dT-primed reverse transcription, amplification, labelling of dye swapped technical replicates and hybridisation to long oligonucleotides microarrays. As a control, RNA from two biological replicates of dissected adult testis plus accessory glands (Testis_rep1, Testis_rep2) was amplified, labelled (dye-swap technical replicate) and hybridised to similar arrays. To help with the spot-finding of the arrays genomic DNA was co-hybridised in some cases (this genomic DNA data was excluded from further analysis). Genes with an intensity level below 200 in at least one channel across the Sperm or Testis set were removed (5579 transcripts present in all three sperm replicates, 5358 transcripts from the testis/accessory gland samples and 4295 transcripts common to both data sets). Then the quantile normalisation was independently applied to the Sperm replicate samples and Testis replicates.

			Action	DRS
	FY-1.raw	Raw
	FY-2.raw	Raw
	MF-1.raw	Raw
	MF-2.raw	Raw
	SPERM-MF-dj.xlsx	Xlsx