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Neurodevelopmental Impact of Prenatal Stress: A Proteomic Analysis of Myelination Disruptions in the Avian Embryo

ABSTRACT: Prenatal stress induced by maternal activation of the hypothalamic-pituitary-adrenal (HPA) axis and subsequent release of glucocorticoids can profoundly affect fetal brain development. This study investigates the molecular mechanisms underlying prenatal stress in Gallus gallus domesticus by analyzing the brain proteome of embryos exposed to corticosterone. Using one of the most comprehensive proteomic analyses of the developing chicken brain to date, in which over 6,500 proteins were quantified, we identified significant myelination deficits as a key pathological feature. These results are consistent with previous research in mammalian models, highlighting the utility of Gallus gallus as a non-mammalian model for studying the effects of prenatal stress on neurodevelopment.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Brain

SUBMITTER: Britta Eggers

LAB HEAD: Katrin Marcus

PROVIDER: PXD055206 | Pride | 2025-10-27

REPOSITORIES: Pride

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Neurodevelopmental Impact of Prenatal Stress: A Proteomic Analysis of Myelination Disruptions in the Avian Embryo.

Gaertner Bela B Morosan-Puopolo Gabriela G Brand-Saberi Beate B Schücke Charmaine C Saberi Darius D Klöster Katharina K Faissner Simon S Marcus Katrin K Gellisch Morris M Eggers Britta B

Developmental neurobiology 20251001 4

Prenatal stress, mediated by elevated glucocorticoid (GC) levels, is a relevant modulator of fetal brain development and a known risk factor for neurodevelopmental disorders. Using the avian embryo as a vertebrate model, we injected corticosterone into the yolk on embryonic day 6 (E6) and assessed neurodevelopmental outcomes at day 14 (E14). Through deep proteomic profiling - quantifying over 6500 proteins - we uncovered a robust molecular signature of stress-induced disruption. Key myelin-assoc ...[more]

PMID: 41088801

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