Dataset Information

Development of LC-FAIMS-MS and its application to lipidomics study of Acinetobacter baumannii infection

ABSTRACT: Here we present the development and application of LC-FAIMS-MS/MS based platform for untargeted lipidomics. The combination of normal phase liquid chromatography (NPLC), which can separate different types of phospholipids based on their head groups, and high-resolution MS (HRMS) offers a powerful qualitative and quantitative workflow for serum lipid profiling.We exploited this NPLC-FAIMS-HRMS to analyze the serum lipid profiles in mice infected intranasally with Acinetobacter baumannii, and developed an MS/MS matching tool to identify the detected lipids.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Serum

SUBMITTER: Jianjun Li

LAB HEAD: Jianjun Li

PROVIDER: PXD055224 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	24H-A1-X230413P00121R22867S08.raw	Raw
	24H-A2-X230413P00121R22867S10.raw	Raw
	24H-A3-X230413P00121R22867S12.raw	Raw
	24H-A4-X230413P00121R22867S14.raw	Raw
	24H-A5-X230413P00121R22867S16.raw	Raw

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Development of LC-FAIMS-MS and its application to lipidomics study of Acinetobacter baumannii infection.

Li Jianjun J Stupak Jacek J Haqqani Arsalan S AS Harris Greg G Zhou Hongyan H Williamson Sam S Chen Rui R Xu H Howard HH Chen Wangxue W

Journal of lipid research 20241010 11

The recent advances in mass spectrometry (MS) technologies have enabled comprehensive lipid profiling in biological samples. However, the robustness and efficiency of MS-based lipidomics is compromised by the complexity of biological samples. High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a technology that can continuously transmit one type of ion, independent of the mass-to-charge ratio. Here we present the development and application of LC-FAIMS-MS/MS-based platform for un ...[more]

PMID: 39395788

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