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Comparative protein profiling of stretched (high) and non-stretched podocytes

ABSTRACT: Podocyte detachment due to mechanical stress is a common issue in hypertension-induced kidney disease. The present study investigated the proteome alterations in mechanically stretched podocytes, a model for glomerular hypertension in comparison to non-stretched cells. Conditionally immortalized differentiated podocytes were seeded on flexible silicon membranes of a six well plate, which was mounted on a manifold connected to a custom-built stretch apparatus (NIPOKA GmbH, Greifswald, Germany), which induced cyclic pressure variations resulting in upward and downward motion of the silicone membranes. Pressure amplitude was chosen to give a maximum up- and downward deflection of the membrane centre of 8 mm (high stretch).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Glomerular Visceral Epithelial Cell

SUBMITTER: Elke Hammer

LAB HEAD: Elke Hammer

PROVIDER: PXD055232 | Pride | 2025-05-13

REPOSITORIES: Pride

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			Action	DRS
	20210407_QEp_AW_Sys_Neph_1_V5_unstretch.raw	Raw
	20210407_QEp_AW_Sys_Neph_2_V7_unstretch.raw	Raw
	20210407_QEp_AW_Sys_Neph_3_V5_stretch.raw	Raw
	20210407_QEp_AW_Sys_Neph_4_V8_stretch.raw	Raw
	20210407_QEp_AW_Sys_Neph_5_V8_unstretch.raw	Raw

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Comparative protein profiling of stretched (low) and non-stretched podocytes

Project description:Podocyte detachment due to mechanical stress is a common issue in hypertension-induced kidney disease. The present study investigated the proteome alterations in mechanically stretched podocytes, a model for glomerular hypertension in comparison to non-stretched cells. Conditionally immortalized differentiated podocytes were seeded on flexible silicon membranes of a six well plate, which was mounted on a manifold connected to a custom-built stretch apparatus (NIPOKA GmbH, Greifswald, Germany), which induced cyclic pressure variations resulting in upward and downward motion of the silicone membranes. Pressure amplitude was chosen to give a maximum up- and downward deflection of the membrane centre of 6 mm (low stretch).

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48-h stretched human periodontal ligament cells

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