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Proteomic analysis of a novel iPSC-derived 3D brain tissue model (3BTM) of Alzheimer’s disease

ABSTRACT: Stem-cell-based in vitro models offer promising potential to elucidate pathomechanisms underlying neurological diseases such as Alzheimer’s disease. However, harnessing this potential is currently impaired by low reproducibility, maturity, and cell-type diversity of models and lack of AD-relevant pathologies. Therefore, we developed a novel 3D cortical tissue model containing neurons, astrocytes, and microglia with high reproducibility, maturity, and viability. Upon knock-in of AD-causing APP mutations we found typical pathologies such as time- and cell-type-dependent accumulation and aggregation of Aβ, increased phosphor-tau levels, and microglia activation. To get an unbiased overview of pathology-related changes, we performed proteome analysis of our cultures containing different cell types at different timepoints.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebral Cortex Neuron, Astrocyte, Microglial Cell

SUBMITTER: Stephan Mueller

LAB HEAD: Stefan Lichtenthaler

PROVIDER: PXD055254 | Pride | 2026-02-23

REPOSITORIES: Pride

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			Action	DRS
	AGDP_2401_01_Slot2-31_1_10905.d.zip	Other
	AGDP_2401_02_Slot2-11_1_10883.d.zip	Other
	AGDP_2401_03_Slot2-18_1_10890.d.zip	Other
	AGDP_2401_04_Slot2-1_1_10873.d.zip	Other
	AGDP_2401_05_Slot2-21_1_10894.d.zip	Other

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Proteomic analysis of a novel iPSC-derived 3D brain tissue model (3BTM) of Alzheimer’s disease

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