Proteomics

Dataset Information

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DNMT3A regulates megakaryocyte-biased HSC fate decisions

ABSTRACT: We characterized the role of the DNA methyltransferase DNMT3A in the fate decisions of murine megakaryocyte-biased HSCs and in the differentiation of megakaryocytes and platelets. In this experiment, we isolated washed platelets from 4 WT and 4 Dnmt3a+/– germline mice (2 males and 2 females per group) and subjected them to proteomic analysis by mass-spectrometry. We sought to identify changes at the protein level that occur in platelets due to aberrant DNA methylation during the differentiation of upstream hematopoietic cells.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Platelet

SUBMITTER: Antrix Jain  

LAB HEAD: Margaret A. Goodel

PROVIDER: PXD055265 | Pride | 2025-06-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
49420_1_EXP_774_mplatlets_WT_male1_2ug.msf Msf
49420_1_EXP_774_mplatlets_WT_male1_2ug.raw Raw
49421_1_EXP_774_mplatlets_WT_male2_1ug.msf Msf
49421_1_EXP_774_mplatlets_WT_male2_1ug.raw Raw
49422_1_EXP_774_mplatlets_Dnmt3a_male1_1ug.msf Msf
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Publications

<h4>Abstract</h4>Hematopoietic stem cells (HSCs) are defined by their capacity to regenerate all main components of peripheral blood, but individual HSCs exhibit a range of preferences for generating downstream cell types. Their propensities are thought to be epigenetically encoded, but few differential regulatory mechanisms have been identified. In this work, we explored the role of DNA methyltransferase 3A (DNMT3A) in the megakaryocyte-biased HSC population, which is thought to reside at the t  ...[more]

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