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Proteomics Faecalicatena sp. DSM22707, Glucose vs Sulfoquinovose

ABSTRACT: Sulfoquinovose (SQ) is a major organosulfonate in nature, and thus plays an important role in the biogeochemical sulfur and carbon cycles. We identified a bacterial anaerobic consortium, enriched from lake Konstanz, which degraded SQ to isethionate as intermediate and further into acetate and sulfide. By a metagenomic analysis we identified Faecalicatena sp. DSM22707 as major SQ-degrader in the consortium. Strain DSM22707 degraded SQ in pure culture into isethionate and small amounts of sulfolactate.

INSTRUMENT(S):

ORGANISM(S): Freshwater Sediment Metagenome Eubacteriaceae Bacterium Peh A

SUBMITTER: Sabrina Borusak

LAB HEAD: David Schleheck

PROVIDER: PXD055296 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	Q2109_SB_210921.csv	Csv
	Q2109_SB_210921_A.msf	Msf
	Q2109_SB_210921_A_103.raw	Raw
	Q2109_SB_210921_A_104.raw	Raw
	Q2109_SB_210921_C.msf	Msf

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Publications

Anaerobic Faecalicatena spp. degrade sulfoquinovose via a bifurcated 6-deoxy-6-sulfofructose transketolase/transaldolase pathway to both C2- and C3-sulfonate intermediates.

Borusak Sabrina S Denger Karin K Dorendorf Till T Fournier Corentin C Lerner Harry H Mayans Olga O Spiteller Dieter D Schleheck David D

Frontiers in microbiology 20241205

Plant-produced sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is one of the most abundant sulfur-containing compounds in nature and its bacterial degradation plays an important role in the biogeochemical sulfur and carbon cycles and in all habitats where SQ is produced and degraded, particularly in gut microbiomes. Here, we report the enrichment and characterization of a strictly anaerobic SQ-degrading bacterial consortium that produces the C2-sulfonate isethionate (ISE) as the major pro ...[more]

PMID: 39712897

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Sulfoglycolysis sustains Eubacterium rectale in low-fiber diets

Project description:The production of short-chain fatty acids by Firmicutes within the human gastrointestinal tract is recognized as critical for gut health and the progression of a range of disease states. Firmicutes lack many glycan-degrading pathways and instead derive a major proportion of their metabolic precursors from carbohydrates released by glycan-degrading generalists belonging to the Bacteroidota phylum and Bifidobacteriaceae family. Recently, it was shown that Eubacterium rectale, a widespread member of the Firmicutes belonging to the Clostridiales cluster XIVa, can grow on the unusual but ubiquitous plant-derived sugar sulfoquinovose (SQ) using a sulfoglycolytic sulfofructose transaldolase pathway. Here, we show that in addition to SQ, E. rectale can also grow on the SQ glycoside sulfoquinovosyl glycerol (SQGro). The 3D structure of the E. rectale sulfoquinovosidase shares strong structural conservation with SQases from gram-negative bacteria. Using sequence-similarity networks, we provide new biological context to a conserved domain of unknown function protein SftX belonging to DUF4867, which is conserved in the sulfoglycolytic sulfofructose transaldolase pathway and determine its 3D structure. Finally, with the aid of a synthetic mini-human microbiome reconstituted in germ-free mice, we show that an SQ dietary supplement can rescue E. rectale from population crashes that occur upon switching from a high-fibre to a low-fibre, high-fat diet. This suggests that SQ or SQGro has potential as a prebiotic for promoting the maintenance of this important SCFA-producing bacterium within the colonic microbiota.

2025-03-04 | PXD053947 | Pride

Pseudomonas putida SQ1 pull-down

Project description:Pseduomonas putida SQ1 cell lysates grown on sulfoquinovose (SQ) were labelled with activity-based probe 3 and labelled proteins pulled-down using streptavidin beads. An on-bead digest was performed and the resulting peptides analysed by LCMS/MS.

2024-07-10 | PXD047091 | Pride

Quantitative proteomics and RNA-sequencing of mouse liver endothelial cells identify novel regulators of BMP6 by iron

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2023-12-28 | PXD039830 | Pride

Global analysis of gene expression by SV40 T antigen in mouse embryo fibroblasts

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Expression data from adipocyte differentiation from diabetic adipose-derived stem cells (dADSC) treated with squalene (Sq) and its derivative (HH-Sq)

Project description:Gene expression profiling reveals functional difference between Sq and HH-Sq on differentiation, metabolism, and lipid droplot formation of dADSC Microarray gene expression profiling was conducted for technical replicates of adipocyte induced dADSC (IN), adipocyte induced and treated with Sq (Sq), adipocyte induced and treated with HH-Sq (HH-Sq) for 14 days (1 µM) to identify its effect in the regulation of adipocyte differentiation, metabolism, and lipid droplet accumulation. Technical replicates represent the same hybridization mixture applied to 3 independent arrays.

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Expression data from SQ, TRI-SQ and Tetra-SQ-treated HFDPCs

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Expression data from SQ, TRI-SQ and Tetra-SQ-treated HFDPCs

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2022-08-25 | GSE211839 | GEO

proximity labeling 4T1 Cells through PCN

Project description:The 4T1 cells were labeled with BxxP via HA-PCN nanozyme as described above. After washing 5 times with PBS, cells were collected and lysed in RIPA buffer containing 1 % (v/v) Protease inhibitor cocktail (ThermoFisher Scientific) and 1 % (v/v) PMSF under bath-sonication, followed by centrifugation for 10 minutes at 4 ℃ to remove cell debris. Before the enrichment experiment, Pierce streptavidin magnetic beads (ThermoFisher Scientific) were washed twice with RIPA buffer for 2 minutes. The 400 μg samples were mixed with 50 μL beads slurry with rotation for 2 hours at 4 ℃, followed by washing twice with 1 mL RIPA buffer for 2 minutes, once with 1 mL 1 M KCl for 2 minutes, once with 1 mL 0.1 M Na(2)CO(3) for 2 minutes, once with 1 mL 2 M urea (pH=7.5) for 2 minutes, twice with RIPA buffer for 2 minutes []. Before enrichment, 100 μL cell lysis was packed as the input sample. For the western blotting analysis, the labeled proteins were eluted from the beads by boiling with protein loading buffer containing 20 mM dithiothreitol (DTT, ThermoFisher Scientific) and 2 mM biotin.

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Single Cell RNA sequencing of mouse SQ tumor from C57BL/6 with or with liver tumor.

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