Proteomics

Dataset Information

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BMP9 Secretome of hHiPC LC-MS/MS

ABSTRACT: To investigate BMP9/ALK1 signaling in human highly proliferative cells (hHiPC), we selected one primary population of hHiPC from 3 individuals and studied the secretome responses to BMP9 treatment. Proteins were collected in hHiPC treated conditioned media (CM) for mass spectrometry analysis. Proteomic analysis of the hHiPC secretome following BMP9 treatment demonstrates that the secreted proteins, sclerostin (SOST), meflin/immunoglobulin superfamily containing leucine rich repeat (ISLR), and insulin-like growth factor binding protein-3 (IGFBP3), are novel downstream targets of BMP9/ALK1-mediated signaling. Consistent with this hypothesis, lentiviral shRNA and pharmacological inhibition of ALK1 in hHiPCs suppressed secretion of SOST, ISLR, and IGFBP3 following BMP9 treatment as measured by secretome LC-MS/MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Progenitor Cell, Primary Cell

SUBMITTER: Michayla Moore  

LAB HEAD: Calvin Vary

PROVIDER: PXD055302 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
BAC-HiPC-CM-B9-B10.swath Other
DDA-BAC-HiPC-CM-B9-007.wiff Wiff
DDA-BAC-HiPC-CM-B9-007.wiff.scan Wiff
DDA-BAC-HiPC-CM-Ctrl3.wiff Wiff
DDA-BAC-HiPC-CM-Ctrl3.wiff.scan Wiff
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Publications

ALK1 Signaling in Human Cardiac Progenitor Cells Promotes a Pro-angiogenic Secretome.

Moore Michayla M   Ryzhov Sergey S   Sawyer Douglas B DB   Gartner Carlos C   Vary Calvin P H CPH  

Journal of cellular signaling 20240101 3

Pro-angiogenic paracrine/autocrine signaling impacts myocardial repair in cell-based therapies. Activin A receptor-like type 1 (<i>ACVRL1</i>, ALK1) signaling plays a pivotal role in cardiovascular development and maintenance, but its importance in human-derived therapeutic cardiac cells is not well understood. Here, we isolated a subpopulation of human highly proliferative cells (hHiPCs) from adult epicardial tissue and found that they express ALK1, a high affinity receptor for bone morphogenet  ...[more]

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