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LiP-MS on DCLK1 Purified from HEK293 cells treated with DCLK1-IN-1

ABSTRACT: Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated DCLK1 Purified from HEK293 cells with the probe DCLK1-IN-1 and staurosporine to profile structural effects. This project is part of the IMI EUbOPEN project.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Viviane Reber

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD055350 | Pride | 2025-04-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200320_human_PK_iRT.fasta	Fasta
	20240128_205103_VR_Ex46_DLCK1_directDIA_Report.tsv	Tabular
	240126_LU1_reber_Ex46_1.raw	Raw
	240126_LU1_reber_Ex46_2.raw	Raw
	240126_LU1_reber_Ex46_3.raw	Raw

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