Proteomic analysis reveals blunted effect of sprint interval training by vitamin C and E treatment in both type 1 and type 2 muscle fibres

ABSTRACT: We hypothesized that SIT causes oxidative stress preferentially in fast-twitch type 2-fibres and that this stress constitutes an important trigger of mitochondrial adaptations towards increased oxidative capacity and hence improved muscular endurance. In addition, we hypothesized that the SIT-induced signalling involves an initial ROS-dependent increase in SR Ca2+ leak followed by adaptive changes in the expression of SR Ca2+-handling proteins. To test these hypotheses, two groups of young males performed three weeks of SIT, one group received antioxidants in the form of high dosage of vitamin C and E and the other placebo. In addition to physiological exercise performance tests, we focus on changes in ROS-, SR Ca2+-, and mitochondria-related gene expression after the initial SIT session and fibre type-dependent protein expression after the last SIT session.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leg Muscle, Extrafusal Muscle Fiber

SUBMITTER: Marta Murgia  

LAB HEAD: Håkan Westerblad

PROVIDER: PXD055360 | Pride | 2025-07-29

REPOSITORIES: Pride