Dataset Information

Proteomic analysis reveals blunted effect of sprint interval training by vitamin C and E treatment in both type 1 and type 2 muscle fibres

ABSTRACT: We hypothesized that SIT causes oxidative stress preferentially in fast-twitch type 2-fibres and that this stress constitutes an important trigger of mitochondrial adaptations towards increased oxidative capacity and hence improved muscular endurance. In addition, we hypothesized that the SIT-induced signalling involves an initial ROS-dependent increase in SR Ca2+ leak followed by adaptive changes in the expression of SR Ca2+-handling proteins. To test these hypotheses, two groups of young males performed three weeks of SIT, one group received antioxidants in the form of high dosage of vitamin C and E and the other placebo. In addition to physiological exercise performance tests, we focus on changes in ROS-, SR Ca2+-, and mitochondria-related gene expression after the initial SIT session and fibre type-dependent protein expression after the last SIT session.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leg Muscle, Extrafusal Muscle Fiber

SUBMITTER: Marta Murgia

LAB HEAD: Håkan Westerblad

PROVIDER: PXD055360 | Pride | 2025-07-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20230523_EXPL4_MaMu_SA_M770_FibersSG_PlacPost_008_1_T2.raw	Raw
	20230523_EXPL4_MaMu_SA_M770_FibersSG_PlacPost_008_1_T2_R2.raw	Raw
	20230523_EXPL4_MaMu_SA_M770_FibersSG_PlacPost_008_4_T1.raw	Raw
	20230523_EXPL4_MaMu_SA_M770_FibersSG_PlacPost_008_4_T1_R2.raw	Raw
	20230523_EXPL4_MaMu_SA_M770_FibersSG_PlacPost_008_4_T2.raw	Raw

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Publications

Antioxidant supplementation blunts the proteome response to 3 weeks of sprint interval training preferentially in human type 2 muscle fibres.

Wyckelsma Victoria L VL Murgia Marta M Kamandulis Sigitas S Gastaldello Stefano S Brazaitis Marius M Snieckus Audrius A Eimantas Nerijus N Pääsuke Mati M Edman Sebastian S Apro William W Andersson Daniel C DC Westerblad Håkan H Venckunas Tomas T

The Journal of physiology 20250528

Sprint interval training (SIT) is a time-efficient type of endurance training that involves large type 2 muscle fibre recruitment. Effective antioxidant supplementation may mitigate positive training adaptations by limiting the oxidant challenge. Our aim was to test whether SIT affects type 2 more than type 1 muscle fibres, and whether the muscular training response is mitigated by antioxidant treatment. Young men performed three weekly SIT sessions (4-6 × 30 s all-out cycling) for 3 weeks while ...[more]

PMID: 40433923

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