Characterizing heterologous protein burden in Komagataella phaffii

ABSTRACT: In this study, we explore the molecular response of various K. phaffii strains that produce either the human insulin precursor or Mambalgin-1, examining changes in transcription and changes in intra- and extracellular protein levels. Our findings provide valuable insights into the molecular mechanisms that regulate the behaviour of K. phaffii production strains under the stress of producing different heterologous proteins. We believe that these results will serve as a foundation for identifying new genetic targets to improve strain robustness and productivity.

INSTRUMENT(S):

ORGANISM(S): Komagataella Pastoris (strain Gs115 / Atcc 20864) (yeast) (pichia Pastoris)

SUBMITTER: Louise Kastberg  

LAB HEAD: Jochen Förster

PROVIDER: PXD055382 | Pride | 2025-03-12

REPOSITORIES: Pride