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Proteome analysis of Bacillus cereus ATCC 14579 and its tspO mutant at late exponential and stationary phases

ABSTRACT: The proteomes of Bacillus cereus ATCC 14579 and its tspO mutant were established by label-free shotgun proteomics for cells harvested at late exponential and stationary phases. The comparative analysis indicated the molecular modifications induced by the phase transition and by the mutation of the gene encoding the transmembrane translocator protein TSPO which is a quorum sensing inhibitor.

INSTRUMENT(S):

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD055392 | Pride | 2025-03-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F231303.dat	Other
	F231303_p0.05.mzid.gz	Mzid
	F231307.dat	Other
	F231307_p0.05.mzid.gz	Mzid
	F231312.dat	Other

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Publications

Exoproteomic Evidence for BcTSPO-Mediated Regulation of Virulence Factors in Bacillus cereus.

Duport Catherine C Armengaud Jean J

Proteomics 20250221 7

Bacillus cereus translocator protein (BcTSPO) is a transmembrane protein that plays a regulatory role in various Bacillus cereus phenotypes, potentially including its virulence. Given that the exoproteome is a major determinant of B. cereus virulence, particularly during the late stages of active growth, its analysis is crucial for understanding the regulatory functions of BcTSPO. In this study, we conducted a comparative analysis of the exoproteomes and cellular proteomes of a Δtspo mutant and ...[more]

PMID: 39981793

Similar Datasets

Analysis of the exoproteomes of Bacillus cereus ATCC 14579 and its tspO mutant at late exponential and stationary phases

Project description:The exoproteomes of Bacillus cereus ATCC 14579 and its tspO mutant were established by label-free shotgun proteomics for cells harvested at late exponential and stationary phases. The comparative analysis indicated the molecular modifications induced by the phase transition and by the mutation of the gene encoding the transmembrane translocator protein TSPO which is a quorum sensing inhibitor.

2025-03-25 | PXD055393 | Pride

Molecular changes in the proteome of Bacillus cereus cells upon mutation of the tspO gene.

Project description:The proteome of Bacillus cereus mutated in the TspO gene was established by shotgun proteomics and compared to the proteome of Bacillus cereus wild type strain (ATCC14579) to characterize the molecular modifications induced by the mutation.

2024-05-02 | PXD048360 | Pride

Bacillus cereus strain 31673

Project description:Comparative Genomic Hybridization. Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols.

2010-06-05 | E-GEOD-568 | biostudies-arrayexpress

Gene induction by ethanol stress in B. cereus ATCC 14579

Project description:The stress response of B. cereus ATCC 14579 is monitored true time, showing an enormous response in gene expression. Keywords: Stress response, comparative transcriptome analysis.

2008-05-11 | GSE9855 | GEO

Overexpression analysis of sigZ of B. cereus ATCC 14579.

Project description:The Bacillus cereus ATCC 14579 alternative σ factor σZ and its putative regulon have been characterized. σZ shows overall similarity with ECF σ factors and sigZ constitutes an operon together with asfZ encoding its putative anti-σ factor. Expression analysis revealed sigZ to be induced by an array of stresses, including exposure to ethanol, alkaline pH and heat shock, and a typical promoter binding site for the sigZ-operon was identified by 5’RACE. Phenotypic characterization of B. cereus ATCC 14579 and its sigZ-deletion strain revealed diminished growth performance and sporulation capacity. The σZ-regulon was successfully established by transcriptome analysis of a nisin inducible sigZ-overexpression strain. Overexpression of sigZ was shown to affect expression of 42 genes, including 33 genes encoding proteins located in the extracytoplasm. The identified σZ regulon contained genes encoding proteins situated in the extracytoplasm involved in cell surface modifications and transport. The regulation of genes encoding cell surface modification proteins implies σZ to be involved in the regulation of interaction of B. cereus ATCC 14579 with its environments, which includes human intestinal cells, possibly influencing its virulence status. Keywords: Comparative transcriptome study

2008-05-11 | GSE9860 | GEO

Multiple roles for CodY in the type strain Bacillus cereus ATCC 14579

Project description:Comparison of the B cereus codY deletion mutant with wild type strain (ATCC 14579)

2012-07-05 | GSE31017 | GEO

Bacillus_anthracis_CGH

Project description:Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols.

2010-06-05 | E-GEOD-341 | biostudies-arrayexpress

Gene induction by ethanol stress in B. cereus ATCC 14579

Project description:The stress response of B. cereus ATCC 14579 is monitored true time, showing an enormous response in gene expression. The wild-type strain of B. cereus ATCC 14579 was cultured to an OD600 of ~0.6. Here the first RNA sample was taken, 0 min. After sampling 4% of ethanol was added (v/v), and samples were taken after 10, 30 and 60 minutes of exposure. Comparisons performed were 0 min - 10 min, 0 min - 30 min, and 0 min - 60 min.

2008-05-11 | E-GEOD-9855 | biostudies-arrayexpress

Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987

Project description:Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities.

2015-10-10 | GSE24620 | GEO

Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 14579

2015-10-10 | GSE24619 | GEO