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Impact of Static Myoblast Loading on Protein Secretion Linked to tenocyte Migration

ABSTRACT: Exercise has been shown to promote wound healing, including tendon repair. Myokines released from exercised muscles are believed to play a significant role in this process. In our previous study, we used an in vitro co-culture and loading model to demonstrate that 2% static loading of myoblasts increased the migration and proliferation of co-cultured tenocytes—two crucial aspects of wound healing. IGF-1, released from myoblasts in response to 2% static loading, was identified as a contributor to the increased proliferation. However, the factors responsible for enhanced migration were not clear. In the current study, we subjected myoblasts in single culture conditions to 2%, 5%, and 10% static loading and performed proteomic analysis on the cell supernatants. Gene Ontology (GO) analysis revealed that 2% static loading induced the secretion of NBL1, C5, and EFEMP1, which are associated with cell migration and motility. Further investigation by adding exogenous recombinant proteins to human tenocytes showed that NBL1 increased tenocyte migration, but not proliferation. This effect was not replicated by treatment with C5 and EFEMP1.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: Shaochun Zhu

LAB HEAD: Ludvig J. Backman

PROVIDER: PXD055477 | Pride | 2026-08-07

REPOSITORIES: Pride

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			Action	DRS
	S00058_Exploris01_SZ_Xin_secretome_10p1.raw	Raw
	S00058_Exploris01_SZ_Xin_secretome_10p2.raw	Raw
	S00058_Exploris01_SZ_Xin_secretome_10p3.raw	Raw
	S00058_Exploris01_SZ_Xin_secretome_2p1.raw	Raw
	S00058_Exploris01_SZ_Xin_secretome_2p2.raw	Raw

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