Dataset Information

Exploring the interactome of Miro2 in hippocampal neural stem cells through TurboID-mediated proximity labeling

ABSTRACT: Mitochondrial Rho-GTPase (Miro), a protein found on the outer mitochondrial membrane, is required for mitochondrial transport, mitochondrial dynamics, and mitochondrial Ca2+ homeostasis. We previously reported that Miro2 plays a role in the survival of hippocampal neural stem (HCN) cells, with effects on mitochondrial morphology and function. Furthermore, we revealed that Miro2 expression level is associated with neurodegeneration in animal models of Alzheimer's disease and patients with dementia. Nevertheless, a comprehensive investigation is still needed; thus, we analyzed the interacting proteins of Miro2 to elucidate its mechanisms in HCN cells by employing TurboID-based proximity labeling.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Stem Cell

SUBMITTER: Chulhwan (Sean) Kwak

LAB HEAD: Heuiran Lee

PROVIDER: PXD055479 | Pride | 2026-02-23

REPOSITORIES: Pride

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Publications

Proximity labeling unveils potential roles of the Miro2-CISD1 network in mitochondrial dynamics and neuronal differentiation.

Kang Im Kyeung IK Lee Sunwoo S Moon Tae Kwon TK Han A Reum AR Yu Dae Bong DB Jung Minkyo M Kwak Chulhwan C Seo Jeong Kon JK Rhee Hyun-Woo HW Kim Seong Who SW Woo Ha-Na HN Mun Ji Young JY Lee Heuiran H

Communications biology 20260209 1

Adult hippocampal neurogenesis, crucial for maintaining neural homeostasis, is integral to neurodegeneration. We previously identified Miro2 as a key regulator of mitochondrial dynamics and survival in hippocampal neural stem cells with potential relevance to Alzheimer's disease. Here, using TurboID-based proximity labeling, we explore Miro2's interaction networks and identify sixty-six unique interactors specific to hippocampal neural stem cells. Functional enrichment analysis reveals that thes ...[more]

PMID: 41663665

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