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Analysis of type IV collagen alpha chain ratios in Caenorhabditis elegans

ABSTRACT: CRISPR / Cas-9 mediated homologous recombination was used to fuse type IV collagen alpha chains (EMB-9 and LET-2) with the fluorescent proteins mNeonGreen and mRuby2 in Caenorhabditis elegans. L1 to L3 larvae were collected and processed for mass spectrometry. The goal of this study was to compare the abundance of each chain. The Caenorhabditis elegans strains used in this study were NK3077(qy228 [let-2::mNG] X ; qy244 [emb-9::mRuby2] III) and NK3083 (qy216 [let-2::mRuby2] X ; qy236 [emb-9::mNG] III).

INSTRUMENT(S):

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Larva

SUBMITTER: Mychel Morais

LAB HEAD: Rachel Lennon

PROVIDER: PXD055575 | Pride | 2026-02-02

REPOSITORIES: Pride

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			Action	DRS
	20240521_LennonR_WilliamsE_11.raw	Raw
	20240521_LennonR_WilliamsE_12.raw	Raw
	20240521_LennonR_WilliamsE_13.raw	Raw
	20240521_LennonR_WilliamsE_14.raw	Raw
	20240521_LennonR_WilliamsE_15.raw	Raw

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