Dataset Information

Interaction with AK2 establishes AIFM1 as organizing platform in mitochondria

ABSTRACT: Interactome of overexpressed, HA-tagged AIFM1 in HEK293T AIFM1 KO background vs control (3797, 3593), Interactome of overexpressed, HA-tagged AIFM1 variants in HEK293T AIFM1 KO background vs control (3859), Comparison of whole cell extract after gelfiltration of HEK293T AIFM1 KO cell line vs WT

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Prerana Wagle

LAB HEAD: Jan Riemer

PROVIDER: PXD055617 | Pride | 2025-08-04

REPOSITORIES: Pride

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Publications

Interaction with AK2A links AIFM1 to cellular energy metabolism.

Rothemann Robin Alexander RA Pavlenko Egor E Mondal Mrityunjoy M Gerlich Sarah S Grobushkin Pavel P Mostert Sebastian S Racho Julia J Weiss Konstantin K Stobbe Dylan D Stillger Katharina K Lapacz Kim K Salscheider Silja Lucia SL Petrungaro Carmelina C Ehninger Dan D Nguyen Thi Hoang Duong THD Dengjel Jörn J Neundorf Ines I Bano Daniele D Poepsel Simon S Riemer Jan J

Molecular cell 20250626 13

Apoptosis-inducing factor 1 (AIFM1) is a flavoprotein essential for mitochondrial function and biogenesis. Its interaction with MIA40/CHCHD4, the central component of the mitochondrial disulfide relay, accounts for some, but not all, aspects of AIFM1 function. We provide a high-confidence AIFM1 interactome that elucidates functional partners within the mitochondrial intermembrane space. We found that AIFM1 binding to adenylate kinase 2 (AK2), an essential enzyme that maintains cellular adenine n ...[more]

PMID: 40578348

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