Dataset Information

Colorectal cancer Circulating tumor cells released Extracelluar Vesicles proteome cargo analysis

ABSTRACT: Background: Circulating tumor cells (CTCs) play a key role in cancer progression, and in vitro CTC models are essential for studying their biology. This study examined extracellular vesicles (EVs) from CTC lines of a metastatic colorectal cancer (mCRC) patient who progressed despite therapy. Methods and results: EVs from different CTC lines showed size and morphological variations. Proteomic analysis revealed an enrichment of proteins linked to stemness, endosomal biogenesis, and mCRC prognosis. Integrins were notably enriched in EVs from post-therapy CTC lines. In vivo, EVs accumulated in the liver, lungs, kidneys, and bones, though their influence on CTC organotropism was unclear. Conclusions: This study highlights therapy-related changes in EVs from mCRC CTCs and their role in cancer spread. These findings suggest the utility of CTC-derived EVs in understanding cancer dissemination, though further validation in mCRC patients is needed.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Malignant Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Luis Enrique Cortes Hernandez

LAB HEAD: Catherine Alix-Panabieres

PROVIDER: PXD055639 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	01Luis_CTC41_1B1_Slot2_21_01_3809.d.zip	Other
	02Luis_CTC41_1B2_Slot2-36_01_3827.d.zip	Other
	03Luis_CTC41_1B3_Slot2-10_01_3844.d.zip	Other
	04Luis_CTC41_1B4_Slot2-25_01_3861.d.zip	Other
	05Luis_CTC41_1B5_Slot2-10_01_3888.d.zip	Other

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Publications

Proteomic profiling and functional analysis of extracellular vesicles from metastasis-competent circulating tumor cells in colon cancer.

Cortés-Hernández Luis Enrique LE Eslami-S Zahra Z Attina Aurore A Batista Silvia S Cayrefourcq Laure L Vialeret Jérôme J Di Vizio Dolores D Hirtz Christophe C Costa-Silva Bruno B Alix-Panabières Catherine C

Journal of experimental & clinical cancer research : CR 20250322 1

<h4>Background</h4>Circulating tumor cells (CTCs) are pivotal in cancer progression, and in vitro CTC models are crucial for understanding their biological mechanisms. This study focused on the characterization of extracellular vesicles (EVs) from CTC lines derived from a patient with metastatic colorectal cancer (mCRC) at different stages of progression who progressed despite therapy (thus mirroring the clonal evolution of cancer).<h4>Methods and results</h4>Morphological and size analyses reve ...[more]

PMID: 40119417

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Project description:Tumor cells dissociate from the primary site and enter into systemic circulation (circulating tu-mor cells, CTCs) either alone or Tumor cells dissociate from the primary site and enter into systemic circulation (circulating tu-mor cells, CTCs) either alone or as tumor microemboli (clusters); the latter having an increased predisposition towards forming distal metastases than single CTCs. The formation of clusters is, in part, created by contacts between cell–cell junction proteins and/or cytokine receptor pairs with other cells such as neutrophils, platelets, fibroblasts, etc. In the present study, we provide evidence for an extravesicular (EV) mode of communication between pancreatic cancer CTCs and neutrophils. Our results suggest that the EV proteome of CTCs contain signaling proteins that can modulate degranulation and granule mobilization in neutrophils and, also, contain tis-sue plasminogen activator and other proteins that can regulate cluster formation. By exposing naïve neutrophils to EVs isolated from CTCs, we further show how these changes are modulated in a dynamic fashion indicating evidence for a deeper EV based remodulatory effect on com-panion cells in clusters.as tumor microemboli (clusters); the latter having an increased predisposition towards forming distal metastases than single CTCs. The formation of clusters is, in part, created by contacts between cell–cell junction proteins and/or cytokine receptor pairs with other cells such as neutrophils, platelets, fibroblasts, etc. In the present study, we provide evidence for an extravesicular (EV) mode of communication between pancreatic cancer CTCs and neutrophils. Our results suggest that the EV proteome of CTCs contain signaling proteins that can modulate degranulation and granule mobilization in neutrophils and, also, contain tis-sue plasminogen activator and other proteins that can regulate cluster formation. By exposing naïve neutrophils to EVs isolated from CTCs, we further show how these changes are modulated in a dynamic fashion indicating evidence for a deeper EV based remodulatory effect on com-panion cells in clusters.