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Analysis of Human Plasma by LC-MSMS and Linear Ion Trap

ABSTRACT: Identification of proteins from normal human plasma by LC-MSMS with linear ion trap after collection over quaternary amine resin and digestion with trypsin.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: John Marshall

LAB HEAD: John G. Marshall

PROVIDER: PXD055704 | Pride | 2025-05-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Plasma_0mMNaCl_Rep1.bioML	Other
	Plasma_0mMNaCl_Rep1.raw	Raw
	Plasma_0mMNaCl_Rep2.RAW	Raw
	Plasma_0mMNaCl_Rep2.bioML	Other
	Plasma_0mMNaCl_Rep3.RAW	Raw

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Publications

Comparison of the Human Plasma Peptides from the Fit of Fragmentation Spectra versus Accurate Monoisotopic Precursor Mass.

Chen Zhuo Zhen ZZ Dufresne Jaimie J Bowden Peter P Marshall John G JG

ACS omega 20250310 11

In nature, ionized peptides with heavy isotopes and hydrogen rearrangements show a broad mass distribution with signals at discrete delta mass values from -3 to +5 Da by mass spectrometry (MS). For many peptides, the intensity of the +1 or +2 Da isotope exceeds the signal from the monoisotopic mass. Therefore, there is a need for a method that improves peptide identification from heavy isotopes or hydrogen rearrangements based on the fit of tandem mass spectra. Peptides may be identified using a ...[more]

PMID: 40160755

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