Dataset Information

Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells

ABSTRACT: Proximity labeling (PL) characterizes protein interactome in living cells. However, exogenous H2O2 required for engineered peroxidase, like APEX2, is cytotoxic, while biotin ligases-based PL have poor temporal resolution. Here, we showed that SOPP3, an engineered photosensitizer, could trigger APEX2 mediated PL within seconds under blue light illumination, without exogenous H2O2 or cytotoxicity. This new PL technology paves an avenue to characterize molecular dynamics of key biomedical events with high spatial-temporal resolution, efficiency, and flexible applicability.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Qian Liu

LAB HEAD: Hu Zhou

PROVIDER: PXD055722 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	20240822_HFX_LiuQian_PWJ_TMT10_group1_F01.raw	Raw
	20240822_HFX_LiuQian_PWJ_TMT10_group1_F02.raw	Raw
	20240822_HFX_LiuQian_PWJ_TMT10_group1_F03.raw	Raw
	20240822_HFX_LiuQian_PWJ_TMT10_group1_F04.raw	Raw
	20240822_HFX_LiuQian_PWJ_TMT10_group1_F05.raw	Raw

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Publications

Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells.

Qu Dajun D Li Yaxin Y Liu Qian Q Cao Biao B Cao Mengye M Lin Xiaoxi X Shen Chengxing C Zou Peng P Zhou Hu H Zhang Wenjuan W Pan Weijun W

Cell research 20241210 2

PMID: 39653757

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