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Phosphoproteomic analysis of control and GCN2KO cells upon glutamine deprivation

ABSTRACT: control and GCN2-knockout HEK293T cells were incubated in glutamine-rich or glutamine-free medium for one hour in four biological replicates

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Frank Stein

LAB HEAD: Aurelio Teleman

PROVIDER: PXD055740 | Pride | 2025-09-08

REPOSITORIES: Pride

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			Action	DRS
	Lara_231120_P2940_JS_GF_FullP_TMT16_R1_FR01.raw	Raw
	Lara_231120_P2940_JS_GF_FullP_TMT16_R1_FR02.raw	Raw
	Lara_231120_P2940_JS_GF_FullP_TMT16_R1_FR03.raw	Raw
	Lara_231120_P2940_JS_GF_FullP_TMT16_R1_FR04.raw	Raw
	Lara_231120_P2940_JS_GF_FullP_TMT16_R1_FR05.raw	Raw

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Publications

mTORC1 senses glutamine and other amino acids through GCN2.

Figlia Gianluca G Müller Sandra S Garcia-Cortizo Fabiola F Neff Marilena M Klinke Glynis G Poschet Gernot G Teleman Aurelio A AA

The EMBO journal 20250721 17

mTORC1 promotes cell growth when nutrients such as amino acids are available. While dedicated sensors relaying availability of leucine, arginine and methionine to mTORC1 have been identified, it is still unclear how mTORC1 senses glutamine, one of its most potent inducers. Here, we find that glutamine is entirely sensed through the protein kinase GCN2, whose initial activation is not triggered by depletion of glutamine itself, but by the concomitant depletion of asparagine. In turn, GCN2 leads t ...[more]

PMID: 40691417

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