Proteomics

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Neutrophils display novel cytosolic proliferating cell nuclear antigen (PCNA) partners involved in interferon response in COVID-19

ABSTRACT: Neutrophils are key players in the hyperinflammatory response during SARS-CoV-2 infection, exhibiting features of deregulation and reprogramming. The cytosolic proliferating cell nuclear antigen (PCNA) is a scaffolding protein highly dependent on the microenvironment status and known to interact with numerous proteins that regulate neutrophil survival, NADPH oxidase-dependent reactive oxygen species (ROS) production, and cell energy metabolism. In this sense this study aimed to examine the cytosolic protein content and PCNA interactome in neutrophils from COVID-19 patients. Methods: Proteomics analysis was performed on neutrophil cytosols from healthy donors and patients with severe or critical COVID-19. In vitro approaches with ultrapure neutrophils were employed to validate the role of PCNA dynamics in neutrophil overactivation during COVID-19.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neutrophil

DISEASE(S): Covid-19

SUBMITTER: Johana Chicher  

LAB HEAD: Véronique Witko-Sarsat

PROVIDER: PXD055810 | Pride | 2025-05-07

REPOSITORIES: pride

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2021_S14_VWitko_10_PC_Basal.mgf Mgf
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2021_S14_VWitko_11_PC_Basal.mgf Mgf
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Publications

<h4>Introduction</h4>Neutrophils are key players in the hyperinflammatory response during SARS-CoV-2 infection. The cytosolic proliferating cell nuclear antigen (PCNA) is a scaffolding protein highly dependent on the microenvironment status and known to interact with numerous proteins that regulate neutrophil functions. This study aimed to examine the cytosolic protein content and PCNA interactome in neutrophils from COVID-19 patients.<h4>Methods</h4>Proteomic analyses were performed on neutroph  ...[more]

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