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C-terminal amides mark proteins for degradation via SCF/FBXO31 – Expression Proteomics

ABSTRACT: FBXO31 is a substrate adapter for the SCF-type ubiquitin ligase complex SCF/FBXO31 which ubiquitylates C-terminal amide-bearing proteins (CTAPs), thereby triggering their proteasomal degradation in human cells. FBXO31(D334N) is a dominant de novo mutation found among patients with cerebral palsy. To identify proteins affected by FBXO31(D334N) expression, we engineered HEK293T cells to carry a FBXO31 knockout and rapidly inducible FBXO31 expression using the Shield-1 degron system. WT or D334N variants of FBXO31 were induced for 12h and proteomic changes captured by whole cell proteomics using TMT-based quantification. Deposited files include raw MS spectra, protein-, peptide- and psm-level identification results, and differential gene expression results.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Matthias Muhar

LAB HEAD: Prof. Jacob Corn

PROVIDER: PXD055818 | Pride | 2024-11-05

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20220818_C29220_002_S408177_TMT9plex_pool_f1_A.raw	Raw
	20220818_C29220_003_S408178_TMT9plex_pool_f2_A.raw	Raw
	20220818_C29220_004_S408179_TMT9plex_pool_f3_A.raw	Raw
	20220818_C29220_005_S408180_TMT9plex_pool_f4_A.raw	Raw
	20220818_C29220_006_S408181_TMT9plex_pool_f5_A.raw	Raw

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Project description:FBXO31 is a substrate adapter for the SCF-type ubiquitin ligase complex SCF/FBXO31 which ubiquitylates C-terminal amide-bearing proteins (CTAPs), thereby triggering their proteasomal degradation in human cells. Immunoprecipitation followed by MS (IP-MS) was performed on HA-tagged cDNA-expressed variants of FBXO31 from HEK293T cells to identify putative clients.

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