Dataset Information

A proteomic approach to identify vaccine candidates based on host cell attachment provides a novel protective Pseudomonas aeruginosa vaccine antigen: cell division protein, FtsZ

ABSTRACT: Pseudomonas aeruginosa is an opportunistic Gram negative pathogen that causes severe nosocomial infections in susceptible individuals due to the emergence of multi-drug resistant strains. There are no approved vaccines against P. aeruginosa infections nor candidates in active clinical development, highlighting the need for novel candidates and strategies. Using a cell-blot proteomic approach, we reproducibly identified 49 proteins involved in interactions with human lung epithelial cells across four P. aeruginosa strains. Among these were cell division protein FtsZ and outer membrane protein OpmH. Escherichia coli BL21 cells overexpressing recombinant FtsZ or rOpmH showed a 66- and 15-fold increased ability to attach to 16HBE14o- cells, further supporting their involvement in host cell attachment. Both antigens led to proliferation of NK and CD8+ cytotoxic T cells, significant increases in the production of IFN-γ, IL-17A, TNF and IL-4 in immunised mice and elicited strong antigen-specific serological IgG1 and IgG2c responses. Immunisation with FtsZ, significantly reduced bacterial burden in the lungs by 1.9-log CFU and dissemination to spleen (1.8-log CFU). The protective candidate, FtsZ, would not have been identified by traditional approaches relying on either virulence mechanisms or sequence-based predictions, opening new avenues in the development of an anti-P. aeruginosa vaccine.

INSTRUMENT(S): Q Exactive HF, Bruker Daltonics timsTOF series

ORGANISM(S): Bacteria Pseudomonas Aeruginosa Pa14

SUBMITTER: Irene Jurado Martin

LAB HEAD: Siobhán McClean

PROVIDER: PXD055823 | Pride | 2025-05-07

REPOSITORIES: pride

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			Action	DRS
	2021_04_06_IJM_A1_Slot1-28_1_4275.d.zip	Other
	2021_04_06_IJM_A2_Slot1-29_1_4276.d.zip	Other
	2021_04_06_IJM_B_Slot1-30_1_4277.d.zip	Other
	2021_04_06_IJM_C_Slot1-31_1_4278.d.zip	Other
	2021_04_06_IJM_D_Slot1-32_1_4279.d.zip	Other

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Proteomic approach to identify host cell attachment proteins provides protective Pseudomonas aeruginosa vaccine antigen FtsZ.

Jurado-Martín Irene I Tomás-Cortázar Julen J Hou Yueran Y Sainz-Mejías Maite M Mysior Margaritha M MM Sadonès Océane O Huebner Johannes J Romero-Saavedra Felipe F Simpson Jeremy C JC Baugh John A JA McClean Siobhán S

NPJ vaccines 20241028 1

Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that causes severe nosocomial infections in susceptible individuals due to the emergence of multidrug-resistant strains. There are no approved vaccines against P. aeruginosa infections nor candidates in active clinical development, highlighting the need for novel candidates and strategies. Using a cell-blot proteomic approach, we reproducibly identified 49 proteins involved in interactions with human lung epithelial cells across f ...[more]

PMID: 39468053

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