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Human A4GNT+THP-1 cell membrane proteins bingding with TFF2 by LC-MS

ABSTRACT: NC or A4GNT+THP-1 cells were incubated with TFF2-3xFlag protein overnight at 4℃, then, co-immunoprecipitation with flag beads. Elute fluid was seperated on SDS-PAGE,and Silver Staining were used to screen the differential protein bands , then,the differential protein band was excised and further identified with LC-MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Weihui Fu

LAB HEAD: Weihui Fu

PROVIDER: PXD055880 | Pride | 2025-09-29

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	229040-1-pep.xlsx	Xlsx
	229040-1-protein.xlsx	Xlsx
	229040-1.pdResult	Other
	229040-1.raw	Raw
	uniprot-Human.fasta	Fasta

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1 - 5 of 5

Publications

Trefoil factor 2 interacts with A4GNT to regulate inflammation to protect against pneumonia during influenza virus infection.

Fu Weihui W Fan Jun J Peng Tianyun T Sun Peng P Yuan Songhua S Zhu Cuisong C Ding Longfei L Zhang Linxia L Zhao Chen C Zhang Shuye S Zhang Xiaoyan X Xu Jianqing J

iScience 20250814 9

Trefoil factor 2 (TFF2) has been shown to reduce inflammation and promote mucosal repair in models of gastric and colonic injury. However, the role of TFF2 in acute respiratory tract infection remains elusive. Here, we demonstrate that TFF2 protects mice against pneumonia in influenza virus infections. <i>In vitro</i> studies have revealed that TFF2 does not directly bind to the previously reported potential receptors but recognizes the terminal GlcNAc-α-1,4-Gal disaccharide of cell surface prot ...[more]

PMID: 40995127

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