Dataset Information

Label free comparison of the protein cargo of extracellular vesicles (EVs) isolated from SC5314 and ecm33∆ mutant cells of Candida albicans after 16 hours of growth in YPD medium

ABSTRACT: Given the tendency to substitute cellular vaccines with acellular ones, and the complete lack of virulence shown by the C. albicans cell wall mutant ecm33∆, we performed the characterization of its EVs and test their possible use as vaccine for invasive candidiasis. Label free proteomics was carried out comparing the protein cargo of extracellular vesicles (EVs) isolated from SC5314 and ecm33∆ mutant cells of Candida albicans after 16 hours of growth in YPD medium.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Candida Albicans (yeast)

TISSUE(S): Cell Culture

SUBMITTER: Concha Gil

LAB HEAD: Concha Gil García

PROVIDER: PXD055891 | Pride | 2025-05-07

REPOSITORIES: Pride

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Publications

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different Candida albicans Extracellular Vesicles.

Martínez-López Raquel R Molero Gloria G Parra-Giraldo Claudia Marcela CM Cabeza Matías Sebastián MS Castejón Guillermo G García-Durán Carmen C Clemente Luis Felipe LF Hernáez María Luisa ML Gil Concha C Monteoliva Lucía L

International journal of molecular sciences 20241230 1

Extracellular vesicles (EVs) from Candida albicans can elicit immune responses, positioning them as promising acellular vaccine candidates. We characterized EVs from an avirulent C. albicans cell wall mutant (ecm33Δ) and evaluated their protective potential against invasive candidiasis. EVs from the yeast (YEVs) and hyphal (HEVs) forms of the SC5314 wild-type strain were also tested, yielding high survival rates with SC5314 YEV (91%) and ecm33 YEV immunization (64%). ...[more]

PMID: 39796100

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Project description:Candida albicans is the major invasive fungal pathogen of humans, causing diseases ranging from superficial mucosal infections to disseminated, systemic infections which are often life threatening. Hematogenously disseminated candidiasis (HDC) has a 47% mortality rate despite current antifungal therapy. An increase in prevalence, as well as an increasing resistance to most of the clinically important antifungal therapies, provides a strong impetus to understand the molecular mechanisms of pathogenesis and the acquisition of drug resistance. This information holds promise to identify novel therapeutic targets. A complete and accurate characterization of how the transcriptome of C. albicans responds to its interaction with cells from the host is an absolute necessity to accomplish this goal. RNA-seq (deep-sequencing of cDNA) provides an unbiased method to define comprehensively and systematically the transcriptome of an organism. We propose a comprehensive characterization of the C. albicans transcriptome in two different human tissue culture models, using RNA-seq. Such a characterization will shed unprecedented light on how fungal pathogens sense and respond to the host environment and how the host tissue responds to fungal invasion. We performed a comprehensive characterization of the C. albicans transcriptome in two different murine models of candidiasis (HDC and oropharyngeal candidiasis (OPC)), and two different human tissue culture models, using RNA-seq. Because the two murine models accurately recapitulate the pathology that is observed in clinical cases of candidiasis, we are confident that gene expression levels will provide an accurate representation of what occurs during the course of an infection in humans. Such a characterization of the in vivo transcriptome will shed unprecedented light on how fungal pathogens sense and respond to the host environment and how the host tissue responds to fungal invasion. PRJNA211732 C. albicans strain SC5314 was used to infect mice in a murine model of systemic candidiasis as well as a murine model of oropharyngeal candidiasis. For the disseminated model, kidneys were harvested at 6 hours, 12 hours, 24 hours and 48 hours after infection. For the oropharyngeal model, tongues were harvest at 1 day, 2 days, 3 days, and 5 days after infection. Total RNA was extracted from all harvested tissues and subject to RNA-seq. We also tested 3 C. albicans mutants in the disseminated model. For the infections with the mutant strains, only 1 timepoint (24 hours post-infection) was analyzed.

Project description:Candida albicans is the major invasive fungal pathogen of humans, causing diseases ranging from superficial mucosal infections to disseminated, systemic infections which are often life threatening. Hematogenously disseminated candidiasis (HDC) has a 47% mortality rate despite current antifungal therapy. An increase in prevalence, as well as an increasing resistance to most of the clinically important antifungal therapies, provides a strong impetus to understand the molecular mechanisms of pathogenesis and the acquisition of drug resistance. This information holds promise to identify novel therapeutic targets. A complete and accurate characterization of how the transcriptome of C. albicans responds to its interaction with cells from the host is an absolute necessity to accomplish this goal. RNA-seq (deep-sequencing of cDNA) provides an unbiased method to define comprehensively and systematically the transcriptome of an organism. We propose a comprehensive characterization of the C. albicans transcriptome in two different human tissue culture models, using RNA-seq. Such a characterization will shed unprecedented light on how fungal pathogens sense and respond to the host environment and how the host tissue responds to fungal invasion. We performed a comprehensive characterization of the C. albicans transcriptome in two different murine models of candidiasis (HDC and oropharyngeal candidiasis (OPC)), and two different human tissue culture models, using RNA-seq. Because the two murine models accurately recapitulate the pathology that is observed in clinical cases of candidiasis, we are confident that gene expression levels will provide an accurate representation of what occurs during the course of an infection in humans. Such a characterization of the in vivo transcriptome will shed unprecedented light on how fungal pathogens sense and respond to the host environment and how the host tissue responds to fungal invasion. PRJNA86065 Two different strains of C. albicans (SC5314 or WO-1) were used to infect two different types of human tissue culture monolayers (HUVECs or OKF6-TERT2). RNA from the infection mixtures were harvested at 90 minutes, 5 hours and 8 hours post infection. Experiments were performed in biological duplicate.

			Action	DRS
	19_44_enero24.mgf	Mgf
	19_44_enero24.mzML	Mzml
	19_44_enero24.mzid.gz	Mzid
	R010319.raw	Raw
	R150219.raw	Raw

Dataset Information

Label free comparison of the protein cargo of extracellular vesicles (EVs) isolated from SC5314 and ecm33∆ mutant cells of Candida albicans after 16 hours of growth in YPD medium

Dataset's files

Publications

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different <i>Candida albicans</i> Extracellular Vesicles.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Label free comparison of the protein cargo of extracellular vesicles (EVs) isolated from SC5314 and ecm33∆ mutant cells of Candida albicans after 16 hours of growth in YPD medium

Dataset's files

Publications

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different &lt;i&gt;Candida albicans&lt;/i&gt; Extracellular Vesicles.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

From High Protection to Lethal Effect: Diverse Outcomes of Immunization Against Invasive Candidiasis with Different <i>Candida albicans</i> Extracellular Vesicles.