Analysis of the interactome of the RLTPR/CARMIL2 protein in CD4+ T cells
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ABSTRACT: We analyzed the interactome of the mouse protein RLTPR (CARMIL2) using an affinity purification method based on the introduction of a One-Strep-tag (OST) sequence in the C-terminus of the bait protein by genetic engineering of primary mouse T cells. We used mouse models expressing the tagged version of either the wild-type protein or the protein bearing the Q575E mutation. Affinity purification of these OST tagged proteins was performed using Streptactin beads, from T cells either non stimulated, stimulated 2min, 5min or 10min with pervanadate. Each AP-MS purification of an OST- protein is associated with a corresponding control (purification from WT CD4+ T cells) in the same conditions of stimulation. Three replicate biological experiments were performed.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Spleen, T Cell
SUBMITTER:
Anne Gonzalez de Peredo
LAB HEAD: Odile Burlez-Schiltz
PROVIDER: PXD055893 | Pride | 2025-05-12
REPOSITORIES: Pride
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