Dataset Information

Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli

ABSTRACT: Peatlands play a crucial role in global climate protection as carbon sinks and their rewetting is increasingly significant but poses economic challenges for agriculture. A promising application for rewetted peatlands is the cultivation of Drosera rotundifolia L., a medicinal plant with potential for sustainable phytopharmaceutical development. The plant's bioactive compounds, particularly flavonoids and naphthoquinones, exhibit biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains, offering new therapeutic options. This study investigates the molecular mechanisms of these compounds in biofilm inhibition through proteomic analyses. Specific fractions of flavonoids and naphthoquinones, as well as individual substances like 7-methyl juglone and 2’’-O-galloyl hyperoside, are analyzed. Results show that flavonoids from Drosera rotundifolia L. likely affect biofilm formation by creating an iron-poor environment through iron complexation and additionally influence polyamine balance, reducing intracellular spermidine levels. Further investigations include assays for iron complexation and analysis of polyamines confirmed the proteomic data. In-silico docking studies identify potential molecular targets of the bioactive compounds. Safety evaluations through cytotoxicity tests in 3D cell cultures and the Galleria mellonella in-vivo model confirmed the safety of the extracts used. These findings highlight Drosera rotundifolia L. as a promising candidate for new phytopharmaceuticals and support the sustainable use of rewetted peatlands.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Sandra Maass

LAB HEAD: Dörte Becher

PROVIDER: PXD055987 | Pride | 2025-05-07

REPOSITORIES: Pride

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			Action	DRS
	220103_OV3_P3_PG_SK_SGe_2022_029_12ul_K_01.raw	Raw
	220103_OV3_P3_PG_SK_SGe_2022_029_12ul_K_02.raw	Raw
	220103_OV3_P3_PG_SK_SGe_2022_029_12ul_K_03.raw	Raw
	220103_OV3_P3_PG_SK_SGe_2022_029_12ul_K_04.raw	Raw
	220103_OV3_P3_PG_SK_SGe_2022_029_12ul_K_05.raw	Raw

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Publications

Drosera rotundifolia L. as E. coli biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies.

Gerschler Sandy S Maaß Sandra S Gerth Philip P Schulig Lukas L Wildgrube Toni T Rockstroh Jan J Wurster Martina M Methling Karen K Becher Dörte D Lalk Michael M Schulze Christian C Guenther Sebastian S Schultze Nadin N

Biofilm 20250228

The successful sustainable cultivation of the well-known medicinal plant sundew on rewetted peatlands not only leads to the preservation of natural populations, but also provides a basis for the sustainable pharmaceutical use of the plant. The bioactive compounds of sundew, flavonoids and naphthoquinones, show biofilm-inhibiting properties against multidrug-resistant, ESBL-producing E. coli strains and open up new therapeutic possibilities. This study investigates the molecular mechanisms ...[more]

PMID: 40124935

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Project description:Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular iron in P. gingivalis affects the expression of a distinct iron-associated regulon of 64 genes and low iron invokes an iron sparing response. Iron homeostasis is usually mediated in Gram-negative bacteria at the transcriptional level by the Ferric Uptake Regulator (Fur). There is a single predicted P. gingivalis Fur superfamily orthologue named Har (heme associated regulator) that lacks the conserved metal binding residues found in other Fur orthologues. We show that Har binds both heme and ferrous iron resulting in a conformational change in the protein. Har was unable to complement the Escherichia coli H1780 fur mutant and there was no change in cellular metal content in a P. gingivalis Har mutant compared with the wild-type. The Har regulon of 44 genes is not predicted to play a role in iron homeostasis. Together these data indicated that Har does not regulate iron homeostasis in P. gingivalis. However, Har was required for heme-responsive biofilm development and its regulon overlapped P. gingivalis regulons previously identified after growth in heme limitation or as a homotypic biofilm. P. gingivalis is unique as an iron-dependent Gram-negative bacterium with a single heme-binding Fur superfamily orthologue, Har, that does not regulate iron homeostasis. Paired samples were compared on the same microarray using a two-colour system. A total of 6 paired microarray hybridizations were performed representing 6 biological replicates, where a balanced dye design was used, with the overall analysis including three microarrays where P. gingivalis 33277 samples were labeled with Cy3 and the paired ECR455 samples were labeled with Cy5 and three other microarrays where samples were labeled with the opposite combination of fluorophores.

Dataset Information

Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli

Dataset's files

Publications

<i>Drosera rotundifolia</i> L. as <i>E. coli</i> biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies.

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Dataset Information

Sustainable Utilization of Rewetted Peatlands: Drosera rotundifolia L. as a Traditional Medicinal Plant for Developing New Phytopharmaceuticals Against Biofilm-Forming Multidrug-Resistant E. coli

Dataset's files

Publications

&lt;i&gt;Drosera rotundifolia&lt;/i&gt; L. as &lt;i&gt;E. coli&lt;/i&gt; biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

<i>Drosera rotundifolia</i> L. as <i>E. coli</i> biofilm inhibitor: Insights into the mechanism of action using proteomics/metabolomics and toxicity studies.