Dataset Information

The butyrate-producing Gram-positive human gut bacterium, Hoskinsella mucinilytica, selectively targets host mucin N-acetylhexosamines

ABSTRACT: The personalized communities of microorganisms (microbiota) that inhabit the distal guts of humans have evolved to process a variety of complex carbohydrates. Many gut bacteria depolymerize and ferment dietary fiber polysaccharides, mutualistically providing short-chain fatty-acids to their host. Some human gut bacteria have evolved to utilize components of host mucin glycoproteins—the major component of protective mucus—and recent studies have implicated specific mucin-degrading commensal bacteria in the development of intestinal inflammation. Thus, identifying new gut bacteria that possess the ability to degrade host mucins and determining how these organisms might cooperate or compete for mucin components during health and disease is an important goal. Here, we used culture on solid medium containing gastric mucin as the main carbon source to isolate a novel bacterium that is largely restricted to using the N-acetylglucosamine and N-acetylgalactosamine sugars found in mucin O-glycans. This butyrate-producing bacterium accesses these sugars from both polymeric gastric mucin and chemically released oligosaccharides and has a genome with correspondingly restricted carbohydrate-active enzyme content. Surprisingly, strains related to this isolate appear to be rare in the now numerous sequence-based microbiota surveys with only 32/3781 (0.85%) human subjects containing this bacterium with an overall relative abundance ranging from 0.004-0.057%. This combination of low prevalence and abundance, suggests that this species could occupy an unknown niche in which access to mucin is important but otherwise render it difficult to detect in mostly feces-based microbiota surveys.

INSTRUMENT(S):

ORGANISM(S): Erysipelotrichaceae Bacterium

SUBMITTER: Nicholas Pudlo

LAB HEAD: Eric C Martens

PROVIDER: PXD056157 | Pride | 2026-04-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PRF_F_2023_E_MART_1103_63779.msf	Msf
	PRF_F_2023_E_MART_1103_63779.raw	Raw
	PRF_F_2023_E_MART_1103_63780.msf	Msf
	PRF_F_2023_E_MART_1103_63780.raw	Raw

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The butyrate-producing Gram-positive human gut bacterium, Hoskinsella mucinilytica, selectively targets host mucin N-acetylhexosamines.

Pudlo Nicholas A NA Pereira Gabriel Vasconcelos GV Schaus Sadie R SR Yang Qinnan Q Fuentes Jaime J JJ Jin Chunsheng C Hein Robert R Zhang Li L Terrapon Nicolas N Lyssiotis Costas A CA Schmidt Thomas M TM Hansson Gunnar C GC Luis Ana S AS Martens Eric C EC

The Journal of biological chemistry 20260317 5

The personalized microbial communities (micobiota) that inhabit the distal guts of humans have evolved to process a variety of complex carbohydrates. Many gut bacteria depolymerize and ferment dietary fiber polysaccharides, mutualistically providing short-chain fatty acids and other metabolites to their host. Some human gut bacteria have evolved to utilize components of host mucin glycoproteins-the major component of secreted mucus that protects the gut. Recent studies have implicated certain mu ...[more]

PMID: 41856288

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