Dataset Information

Protein ‘Purity’, Proteoforms, and the Albuminome: Critical Observations on Proteome and Systems Complexity

ABSTRACT: The identification of effective, selective biomarkers and therapeutics is dependent on truly deep, comprehensive analysis of proteomes at the proteoform level. Using bovine serum albumin (BSA) isolated by two different protocols, cold ethanol fractionation (CEF) and heat shock fractionation (HSF), the data highlight several concerns regarding proteome analyses using currently popular analytical approaches and what it means to purify a ‘protein’ if the isolate consists of a wide variety of proteoforms and/or co-purifying species. Failure to widely recognize and accept proteome complexity has likely delayed the identification of effective biomarkers and new, more selective drug targets. Integrative top-down proteomics (iTDP) is the most logical available analytical technique to effectively provide the necessary critical depth and breadth for complex proteome analyses. Routine analyses at the level of proteoforms will provide the much-needed data for the development and validation of selective biomarkers and drugs, including biologics.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Breyer-Lynne Woodland

LAB HEAD: Matt Padula

PROVIDER: PXD056316 | Pride | 2024-12-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BSA_CEF_PeaksStudio_Search_Exports.xlsx	Xlsx
	BSA_HSF_PeaksStudio_Search_Exports.xlsx	Xlsx
	BSA_combined_PeaksStudio_Search_Exports.xlsx	Xlsx
	BW20231208_C1.raw	Raw
	BW20231208_C10.raw	Raw

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Publications

Protein "purity," proteoforms, and the albuminome: critical observations on proteome and systems complexity.

Woodland Breyer B Coorssen Jens R JR Padula Matthew P MP

Frontiers in cell and developmental biology 20241210

<h4>Introduction</h4>The identification of effective, selective biomarkers and therapeutics is dependent on truly deep, comprehensive analysis of proteomes at the proteoform level.<h4>Methods</h4>Bovine serum albumin (BSA) isolated by two different protocols, cold ethanol fractionation and heat shock fractionation, was resolved and identified using Integrative Top-down Proteomics, the tight coupling of two-dimensional gel electrophoresis (2DE) with liquid chromatography and tandem mass spectrome ...[more]

PMID: 39720005

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