In depth profiling of the cancer proteome from the flowthrough of standard RNA-preparation kits for precision oncology

ABSTRACT: Cancer is a highly heterogeneous disease, even within the same patient. Biopsies taken from different regions of a tumor may stand in stark molecular contrast to each other. Therefore, the ability to generate meaningful data from multiple platforms using the same biopsy is crucial for translating multi-omics characterizations into the clinic. However, it is generally a cumbersome and lengthy procedure to generate DNA, RNA and protein material from the same biopsy. The Qiagen AllPrep kit is an accessible, straightforward, and widely used kit in clinics worldwide to process biopsies and generate genomic and transcriptomic data from tumors. We aimed to determine if high-quality proteomics data could also be obtained from the remaining material. Here, we develop a procedure for generating deep and quantitatively accurate proteomic information in high throughput from AllPrep flowthrough. With a number of refinements, we obtain in excess of 9,000 quantified proteins, achieving a substantial coverage of the total proteome. Additionaly, we successfully characterize the signaling state of the tumors using phosphoproteomics. Combining a standard kit with in-depth proteomics will be an attractive approach for clinics seeking to implement multi-omics-based precision oncology.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Filip Mundt  

LAB HEAD: Matthias Mann

PROVIDER: PXD056337 | Pride | 2026-10-05

REPOSITORIES: Pride